细胞在组织工程血管中的抗钙化作用.docVIP

  细胞在组织工程血管中的抗钙化作用  邢建洲1，王志维2，高尚志2，李罗成2，张  敏2，邓宏平2** （1. 武警广东总队医院心血管外科，广州 510507； 5  摘要：目的 2. 武汉大学人民医院心血管外科，武汉 430060） 研究利用去细胞猪股动脉管道和犬血管壁细胞构建的组织工程血管植入犬体内 1 年后的变化。方法 12 只成年犬，体重 15～25 公斤，随机分为 2 组：支架组（n=6）为去 细胞猪股动脉管道植入犬股动脉，再细胞化组（n=6）为体外诱导培养的血管壁细胞种植去 细胞猪股动脉管道并预适应后植入犬股动脉，1 年后测定移植动脉的组织钙含量和病理学变 10 化。结果 2 组管道钙含量显著高于自体股动脉，支架组钙含量高于再细胞化组和自体股 动脉；扫描电镜及病理学检查显示：2 组管道内表面均已内皮化， 支架组有散在炎性细胞 浸润。结论 去细胞猪股动脉管道以自体细胞再细胞化有助于降低组织钙沉积。 关键词：组织工程；血管；钙含量；细胞 中图分类号：R496 15 Cells can protect against calcification in a tissue-engineered vessel Xing Jianzhou1, Wang Zhiwei1, Gao Shangzhi2, Li Luocheng2, Zhang Ming2, Deng Hongping2 20 25 30 35 40 (1. Department of Cardiovascular Surgery, Guangdong Provincial Corps Hospital of Chinese Peoples Armed Police Force, Guangzhou 510507; 2. Dept. of Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan 430060) Abstract: O Object To study cell protecting against arterial cacification effects in a tissue-engineered vessel. Methods 12 dog were divided into a scaffold group(n=6) and a recellularizated scaffold group(n=6) at random. Six dogs in the scaffold group underwent implantation of decellularizated porcine artery in femoral. Six dogs in the recellularizated scaffold group underwent implantation of the recellularizated scaffolds in femoral that was established by implantation of bone cells on decellularizated porcine artery and preconditioning in vitro. Morphological changes of the implanted valve and calcium content in the explanted tissue-engineered vessel were evaluated by measuring size of the conduits, light microscopy, OCPC, and scanning. Results Compared with autologous artery and the recellularizated scaffold group, the calcium content of the explanted tissue-engineered vessel is highest in the scaffold group. The calcium content of autologous artery is least. Pathology examination showed that the all tissue-engineered vessels implanted were covered with native vascular endothelial cells, and filled with fibroblasts in b