帕金森病体外细胞模型的研究进展.docVIP

  帕金森病体外细胞模型的研究进展# 范振群，刘树民，周世慧** （黑龙江中医药大学中医药研究院） 5 10 15 20 25 30 35 40 摘要：帕金森病作为一直困扰中老年人的重大疾病，越来越多地受到人们的关注。在帕金森 病的研究过程中，体外细胞模型常被用来进行相关病理、生理及药物干预研究。一般多采用 N-甲基-4-苯基吡啶离子、6-羟基多巴胺或鱼藤酮作为造模剂，来诱导细胞，并通过将野生型 及突变型α-突触核蛋白表达基因转染至细胞内，使其过表达，从而制作出更接近病理实际 的帕金森病细胞模型。同时在细胞的应用上，现在广泛采用的 PC12 细胞系和 SH-SY5Y 细 胞系各有各自的特点，不同细胞系可能会使实验产生不同结果，并且同一细胞系中细胞的分 化与否也关系着实验的结果。因此，在现代帕金森病体外研究中，有多种因素制约着模型建 立，并最终影响实验研究及结果 关键词：帕金森病；细胞模型；N-甲基-4-苯基吡啶离子；鱼藤酮；人神经母细胞瘤细胞 中图分类号：R285.5 Research Advance of Parkinson’s disease cell model fanzhenqun, liushumin, zhoushihui (Institute of Traditional Chinese Medicine,Heilongjiang University of Traditional Chinese Medicine) Abstract: Parkinson’s disease(PD), which serious endangers the health of middle- aged and elderly pepole, has attracted more and more attention in these years. In the study of PD, a lot of in vitro models have been used to reflect the pathological changse and the efficacy of new medicine. In order to establish cell culture models of PD, MPP+, 6-OHDA and rotenone have been used to induce the cell so that they can display different pathologic features of PD. α -synuclein，as a important marker of PD, has been transfected into the cell and overexpress α -synuclein so that the cell model is more similar to the real pathological cell of PD. Different cells lines also affect the experimental result, now PC12 and SH-SY5Y have been widespread used , and they all have their own characteristics.The same cell line also can be divided by some chemical substance, and the divided cell has some special characters which also maybe inflect the experimental result.So above, there are many influencing factors that will affect the establishment of the cell model and then inflect the final result. Key words: Parkinson; cell model; MPP+; rotenone; SH-SY5Y 0 引言 帕金森病(Parkinson`s disease,PD)，又称作震颤麻痹症，由英国医生詹姆斯·帕 金森在 1817 年首次发现。每年在 40 岁以上的人群中大约有 0.1%被这种疾病所 困扰[1]。到现在为止发现，PD 的病因仍未被明确，但一般认为主要是由于中枢 神经系统受损所引起，其主要的病理过程是黑质中多巴胺能神经元的缺失和黑质 纹状体通路的损伤。而以上这些特征又不能完全解释 PD 的发病机制和过程，多 种脑内神经的病