石菖蒲活性成分配伍对快速老化小鼠学习记忆功能的影响.docVIP

  石菖蒲活性成分配伍对快速老化小鼠学习 记忆功能的影响# 林雅萍1，陈云波1，魏刚2，刘玉1，田会娟1，程淑意1， 5 10 15 20 25 30 35 40 王奇1，梁伟雄1，陈东风3** （1. 广州中医药大学临床药理研究所 DME 中心, 广东广州 510405； 2. 广州中医药大学新药开发研究中心，广东广州 510006； 3. 广州中医药大学基础医学院，广东广州 510006） 摘要：目的：观察不同剂量的石菖蒲活性成分配伍药液（β-细辛醚:丁香酚=4:1）对快速老 化小鼠（SAMP8）学习记忆功能的影响。方法：将 SAMP8 随机分为模型组，阳性对照药石 杉碱甲组，低剂量配伍组，高剂量配伍组；抗快速老化小鼠（SAMR1）作为正常对照组。 除正常对照组、模型组给予相同体积饮用水外，其他组小鼠给予相应药物治疗，灌胃 2 个月； 给药结束后次日进行 Morris 水迷宫测试，以游泳时间和游泳路程为观察指标。结果：与正 常对照组相比，模型组的平均游泳时间和游泳路程均显著延长（P0.01）；与模型组相比， 低剂量配伍组的平均游泳时间和游泳路程均明显缩短（P0.01，P0.05）结论：低剂量的石 菖蒲活性成分配伍组能有效改善快速老化小鼠的学习记忆功能。 关键词：石菖蒲；β-细辛醚；丁香酚；阿尔茨海默病；快速老化小鼠 中图分类号：R 749.1+6 Influnce of effective chemical combination in acorus tatarinowii on learning-memory ability in SAMP8 LIN Yaping1, CHEN Yunbo1, WEI Gang2, LIU Yu1, TIAN Huijuan1, CHENG Shuyi1, WANG Qi1, LIANG Weixiong1, CHEN Dongfeng3 (1. DME Center, Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China; 2. New Drug Research Development Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, China; 3. School of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, China) Abstract: Object: to observe the influence of effective chemical combination of β-asarone and eugenol (4:1) in Acorus Tatarinowii Schott on learning-memory ability in SAMP8. Methods: 6-month-old SAMP8 were randomly divided to 4 groups（12-13 in each group）: SAMP8 model group, huperzine A group, low and high-dose groups of effective chemical combination; SAMR1 as normal control group. After medication for 60 days, mice were subjected to Morris water maze tests for swimming time and distance. Results: compared with SAMR1 group, SAMP8 model group showed prolonged swimming time and distance（P0.01）. Compared with SAMP8 model group, the swimming time and distance in the low-dose groups of effective chemical combination were significantly decreased（P0.01，P005）. Result: low-dose of effective chemical combination in the ratio of 4:1 could improve learning and