水体Cu2+胁迫对克氏原螯虾（Procambarus clarkii）体液免疫功能的影响.docVIP

  水体 Cu2+胁迫对克氏原螯虾（Procambarus clarkii）体液免疫功能的影响# 魏克强，赵婷* 5 10 15 20 25 30 35 40 （山西大学生命科学学院，太原 030006） 摘要：为研究水体中不同浓度的 Cu2+对克氏原螯虾体液免疫功能的影响，分别采用 0(对照)、 0.5、1.0、3.0、5.0、10.0 mg/L Cu2+刺激克氏原螯虾，24 h、48 h、72 h 和 96 h 后测定其血清 的抗菌活力 Ua、溶菌活力 Ub、酚氧化酶活力 PO 以及超氧化物歧化酶 SOD、过氧化氢酶 CAT、丙二醛 MDA 的活性。结果表明，与对照组相比，1.0 、3.0、 5.0 和 10.0 mg/L 组分 别在染毒 72h 和 48h 后，血清 Ua 活力和 Ub 活力显著下降（P ≤ 0.05）；PO 活性的变化 存在时间-剂量的效应关系，96h 后各处理组均显著降低（P ≤ 0.05）；3.0、5.0 和 10.0mg/L 组的 SOD 和 CAT 活力，与对照组相比在 72h 后均呈现出显著的抑制状态；丙二醛(MDA) 含量随胁迫时间的延长而不断增加，水体 Cu2+浓度越高，MDA 在体内累积量越大。结果提 示，水环境中重金属 Cu2+对克氏原螯虾的体液免疫因子活性具有显著的抑制效应，对机体 健康状况构成潜在的威胁。 关键词：Cu2+；克氏原螯虾；血淋巴；免疫因子 中图分类号：S917 X503.2 Effect of copper on the humoral immunity of Procambarus clarkii WEI Keqiang, ZHAO Ting (School of Life Science, Shanxi University,Taiyuan 030006, China) Abstract: In order to study the effects of copper on the humoral immunity of crayfish Procambarus clarkii, crayfish were exposed to different concentrations of copper at 0, 0.5, 1.0, 3.0, 5.0, 10.0 mg/L. Antibacterial activity (Ua), bacteriolytic activity (Ub), phenol oxidase (PO), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were determined after 24 h, 48 h, 72 h and 96 h of exposure, respectively.The results showed that the activities of Ua and Ub were lower than that in the control group (P ≤ 0.05) after 48h and 72h, when crayfish were exposed to the concentrations of 1.0, 3.0, 5.0 and 10.0 mg/L. PO activity reflected the time-dose response relationship, and PO of each treatment group was significantly lower than that of the control after 96h. Activities of SOD and CAT in 3.0, 5.0 and 10.0 mg/L groups was inhibited significantly during entire experimental period, but MDA in each tested group was higher than that of the control after 48h of exposure (P ≤ 0.05). The results suggested that Cu2+ in water had a significant negative effect on the humoral immunity of Procambarus clarkii, which indicated that heave metal pollution had potential threat to the health of crayfish.