基于纳米粒子等离子体共振耦合的细胞中分子组装的动态...毕业设计（论文）.doc

Dynamic Imaging of Molecular Assemblies in Live Cells Based on Nanoparticle Plasmon Resonance Coupling 基于纳米粒子等离子体共振耦合的细胞中分子组装的动态影像 Jesse Aaron,? Kort Travis,? Nathan Harrison,? and Konstantin Sokolov*,?,§ Department of Biomedical Engineering, Department of Physics, UniVersity of Texas at Austin, Austin, Texas 78712, and Department of Imaging Physics, UT MD Anderson Cancer Center, Houston, Texas 77030 Received June 8, 2009; Revised Manuscript Received July 21, 2009 ABSTRACT　摘要 We used molecular-specific gold nanoparticles to monitor epidermal growth factor receptors (EGFR) in live A431 cells over time. Dark-field hyperspectral imaging, electron microscopy, and electrodynamic modeling were used to correlate optical properties of EGFR-bound plasmonic nanoparticles with receptor regulation state. We showed that receptor trafficking resulted in a progressive red shift of greater than 100 nm in the nanoparticle plasmon resonance wavelength over a time period of 60 min. Furthermore, we demonstrated that changes in peak scattering wavelengths of gold nanoparticles from 546?15 to 574? 20, and to 597?44 nm are associated with EGFR trafficking from the cell membrane, to early endosomes, and to late endosomes/multivesicular bodies, respectively. Finally, we used the changes in scattering spectra of EGFR -bound nanoparticles and a straightforward statistical analysis of RGB-channel color images of labeled cells to create near real-time maps of EGFR regulatory states in living cells. 在A431活细胞中我们用含有特定的金纳米粒子的分子去监控表皮生长因子受体(EGFR)。用暗场高光谱成像、电子显微、电动模拟等手段将已绑定了等离振子纳米颗粒的EGFR的光学属性与受体的调控状态联系起来。在纳米粒子等离振子共振超过60分钟的条件下，我们显示了受体的运输导致了大于100纳米的红移。再者,我们证实，金纳米粒子巅峰散射波长的变化(从546±15到574±20，到597±44 nm)与EGFR的运输有关（分别从细胞膜到早期胞内体，到晚期胞内体/多泡体）。最后, 利用绑定纳米粒子的EGFR的散射光谱的变化以及标定细胞的三基色图像的一个直观数据分析，我们制作了活细胞中接近实时的EGFR调控状态的图像。 Detecting and monitoring the vast number of biomolecular interactions in the cell is a central effort in biology, as these interactions largely govern the behavior of nearly all cell types. Imaging m