生物活性材料支架诱导成年大鼠神经再生修复脑损伤的研究①.pdfVIP

- 318 - 中国康复理论与实践2013 年4 月第19卷第4 期Chin J Rehabil Theory Pract, Apr. 2013, Vol. 19, No. 4 DOI: 10.3969/j.issn. 1006-9771.2013.04.002 ·基础研究· 生物活性材料支架诱导成年大鼠神经再生修复脑损伤的研究① 1 1 2 1 1 陈兰 ，孙敏 ，张皑峰 ，杨朝阳 ，李晓光 [摘要] 目的 应用生物活性材料支架诱导神经再生修复成年大鼠创伤性脑损伤。方法 52 只成年雄性Wistar 大鼠分为假手术 组、单纯损伤组、单纯壳聚糖组和生物活性材料支架组，分别于术后3 d、7 d、2 周、4 周、2 个月和3 个月通过组织化学、免疫组 织化学和神经示踪方法检测神经再生和损伤区新生神经元的来源。结果 应用生物活性材料支架后，海马齿状回(DG)和室管膜下 + + + + + + + + + 区(SVZ) 中BrdU /Nestin 和BrdU /Dcx 细胞较单纯壳聚糖组和单纯损伤组明显增加。损伤区内BrdU /Nestin 、BrdU /Dcx 及BrdU / + + + MAP2 神经细胞较单纯壳聚糖组和单纯损伤组明显增加。DiI 标记SVZ 细胞后，损伤区中见DiI /NeuN 神经元。结论 生物活性材 料支架激活DG 和SVZ 的神经干细胞，SVZ 中的神经干细胞迁移到损伤区，并分化为成熟神经元。 [关键词] 脑损伤；生物活性材料支架；神经干细胞；神经再生；迁移；分化；大鼠 Repair of Injured Adult Rat Brain by Inducing Neural Regeneration with Bioactive Material Scaffolds CHEN Lan, SUN Min, ZHANG Ai-feng, et al. Department of Neurobiology, Capital Medical University, Beijing 100069, China Abstract: Objective To repair the brain of adult rats with traumatic brain injury by inducing neural regeneration with biological scaf- folds. Methods 52 adult male Wistar rats were divided into control group, lesion group, blank chitosan carriers group and bioactive material scaffolds group. The neural regeneration and the origin of the newborn neurons in the injury area were detected through histochemistry, im- mumochemistry and neural tracing on the 3rd day, 7th day, 2nd week, 4th week, 2nd month and 3rd month after operation. Results After ap-