壳聚糖负载硒代胱氨酸纳米粒子的制备及其体外抗肿瘤活性的研究_毕业论文.docx

壳聚糖负载硒代胱氨酸纳米粒子的制备及其体外抗肿瘤活性的研究摘 要硒代胱氨酸(SeC)是一种天然的含硒氨基酸，研究证明，SeC作为一种新型高效的抗肿瘤药物在肿瘤化学预防与治疗中发挥着重要的作用。然而，SeC进入肿瘤细胞与其作用需要较长一段时间，细胞渗透性较差。高分子载药纳米粒子是近年来发展起来的新剂型，药物经其包裹后，不仅可以提高药物稳定性，而且还可以大大提高药物的生物利用率。壳聚糖(CS)是一种优良的天然药物载体，具有良好的生物相容性，生物可降解性和无毒性,被广泛地用作医药载体。本文以壳聚糖为载体，戊二醛为交联剂，液体石蜡为乳化体系，制备出粒径小，分散性好，载药率及包封率较高的SeC壳聚糖纳米粒子。最后通过细胞毒性实验，表明以壳聚糖纳米粒子为药物载体能够增强SeC对MCF-7乳腺癌细胞的抑制作用，并增强其体外抗肿瘤活性。关键词：壳聚糖纳米粒子；乳化交联法；硒代胱氨酸；体外抗肿瘤活性Preparation and in vitro anticancer activity of Selenocystine-loaded chitosan nanoparticlesAbstractSelenocystine (SeC), a naturally occurring selenoamino acid, has been shown to be a novel compound with broad-spectrum anticancer activity. However, it takes a long time for SeC to interact with cancer cells due to its poor solubility and stability. Polymer, a new drug carrier, is widely used in pharmaceutical field. The encapsulation of drug helps to improve drug stability as well as anticancer activity. Chitosan is a popular drug carrier due to its biocompatible, biodegradable and nontoxic properties. In this paper, Chitosan loaded SeC nanoparticles were prepared in emulsion by using glutaraldehyde as crossing agent, span80 as emulsifier and paraffin as oil phase. Lastly, it’s proved that chitosan nanoparticles loaded SeC had greater toxicity and antitumor activity to MCF-7.Key words: Chitosan nanoparticles; cross-linking emulsification; Selenocystine; in vitro anticancer activity 目 录摘 要IAbstractII第一章 绪论31.1 硒抗癌药物的研究进展31.2 硒代胱氨酸的研究现状31.2.1 硒代胱氨酸的性质31.2.2 硒代胱氨酸的抗癌作用31.3 纳米药物载体在肿瘤治疗中的应用31.3.1 纳米技术的研究进展31.3.2 纳米药物载体的研究进展31.4 壳聚糖纳米粒子在纳米药物载体领域的制备和应用31.4.1 壳聚糖的结构与性质31.4.2 壳聚糖纳米粒子的制备31.4.4 壳聚糖纳米粒子的应用31.5 课题的研究意义与创新点31.5.1 课题的研究意义31.5.2 创新点3第二章 空白壳聚糖纳米粒子的制备32.1 实验部分32.1.1 材料与试剂32.1.2 实验仪器32.1.3 实验方法32.2 实验结果与讨论32.4.1 油相体系对壳聚糖纳米粒子的影响32.4.2 油水比对壳聚糖纳米粒子的影响32.4.3 表面活性剂用量对壳聚糖纳米粒子的影响32.4.4 壳聚糖浓度对壳聚糖纳米粒子的影响32.4.5 交联剂用量对壳聚糖纳米粒子的影响32.5 本章小结3第三章 壳聚糖纳米粒子负载硒代胱氨酸的制备33.1 实验部分33.1.1 实验材料33.1.2 实验仪器33.1.3 实验方法33.2 实验结果与讨论33.2.1 粒径分布及其稳定性的分析33.2.2 TEM分析33.2.2 包封率与载药率的计算33.3 本章小结3第四章 细胞毒性的研