SLP-2基因在乳腺癌细胞系中的表达及对MCF-7细胞增殖的影响.docVIP

  SLP-2 基因在乳腺癌细胞系中的表达及对 MCF-7 细胞增殖的影响# 谢贵娥，黄招琴，李莉，徐甜，张航，韩梅，向双林** 5 10 15 20 25 30 35 40 （湖南师范大学生命科学学院，长沙市 410006） 摘要：乳腺癌是目前常见的女性的恶性肿瘤，其发生、演进、侵袭和转移是一个多基因参入 的渐进积累的过程，涉及到许多基因调控、表达、突变等变化。人的 SLP-2 基因是一个新的 肿瘤相关基因，在多种肿瘤的发生过程中发挥重要作用，特别是其在乳腺癌组织的高表达与 病人的存活率相关。因此为了进一步研究 SLP-2 在乳腺癌中的功能，应用免疫印记的方法检 测 SLP-2 在不同乳腺癌细胞中的蛋白表达水平，发现随着细胞癌化程度的增强其表达水平也 增强，并且成功构建 pCMV-Myc-SLP-2 重组质粒，证明了其在 MCF-7 细胞的表达，MTT 实验结果表明 SiRNA 转染乳腺癌细胞 MCF-7 可以抑制细胞增殖，同时我们也发现 SLP-2 在 乳腺癌细胞系中表达量与 HER2/nue 呈负相关。这些结果都为深入研究乳腺癌的发生发展奠 定了基础。 关键词：乳腺癌；SLP-2；免疫印记；细胞增殖 中图分类号：Q78 The expression of SLP-2 in human mammary cancer cell lines and its effect on cell proliferation in MCF-7 cells XIE Guie, HUANG Zhaoqin, LI Li, XU Tian, zhanghang, HAN Mei, XIANG Shuanglin (College of Life Science ,Hunan Normal University ,Changsha 410006) Abstract: Breast cancer is locally advanced at the time of diagnosis,and its occurrence, evolution, invasion and metastasis is a gradual accumulation process of multi-genes participating, it also involves gene regulation, expression, mutation and many other changes. Human stomatin like protein 2 (SLP-2) is a novel cancer-related gene,it played important roles in a variety of tumors, especially high-level SLP-2 expression were associated with significantly decreased survival in breast cancer.To study the function of SLP-2 in breast cancer furtherly,we detected the expression of SLP-2 protein in different breast cancer cells by Western blotting,and found that the expression of its protein in breast cancer cells increased with the increase of the degree of cancer.We also successfully constructed recombinant plasmid pCMV-Myc-SLP-2 and demonstrated it can expressed well in MCF-7. The MTT assay showed that the cell proliferation was significantly inhibited after transfection of pCMV-Myc-SLP-2, we also found that the expression of SLP-2 and HER2/nue have negative correlation in MCF-7, thus providing a useful clue for further research on the occurring and development of breast cancer. Key words: :breast can