快捷的定点突变效率近100的大引物PCR方法.pdfVIP

生物化学与生物物理进展 Progress in Biochemistry and Biop hys ics 2009, 36(11): 1490~ 1494 Fast and Almost 100 % Efficiency Site directed Mutagenesis by The Megaprimer PCR Method* XIE Zhen-Hua1)**, SHI Xiao-Jun2) (1) The Key Laboratory of Chemical Biology, Guangdong Province, Graduate School at Shenzhen, Ts inghua University, Shenzhen 5 18055, China; 2) L ife Sc ience and Ocean Biology Laboratory, Graduate School at Shen