实时荧光定量PCR在植物病害中应用.pdfVIP

中国农学通报 2009,25(07):52-56 Chinese Agricultural Science Bulletin 实时荧光定量PCR 在植物病害中的应用 徐小刚，刘雅婷 （云南农业大学农学与生物技术学院，昆明650201） 摘 要：实时荧光定量PCR 技术作为一项新兴技术，越来越受到人们的重视。它以快速、准确定量、便捷 等优点广泛应用于科学研究各个领域，近年来，实时荧光定量PCR 技术在植物病害研究上不断深入，大 大提高了植物病害的检测效率和监测防治水平。结合了最近几年来应用于检测和鉴定植物病原病害的 实时荧光定量PCR 过程中引物与探针的设计、反应过程、结果评价情况，综合论述了实时荧光定量PCR 技术在由真菌、细菌、病毒、线虫引发的植物病害的检测与应用。 关键词：实时荧光定量PCR ；Ct 值；植物病害；引物设计；灵敏度 中图分类号：S432.4+1 文献标识码：A Application of Real-time Fluorescence Quantitative PCR in Plant Disease Xu Xiaogang, Liu Yating (Yunnan Agricultural University in Agriculture and Biotechnology Institute, Kunming 650201) Abstract: As an emerging technology, more and more people pay attention to the real-time fluorescence quantitative PCR. It has been widely used in different research areas, since it is fast, accurate, and easy use. In recent years, with the developing and more detailed of the technology, it has greatly improved the efficiency of detection and prevention and treatment in plant disease. In this review, it was introduced that real-time fluorescence quantitative PCR detects plant disease infected by the fungus, bacterial virus and nematode, the design of primers and probes, the reactions and the evaluation of real-time fluorescence quantitative PCR. Key words: real-time fluorescence quantitative PCR, Ct, plant disease, primer design, sensitivity 实时荧光定量 PCR （Real-time fluorescence 人们利用实时荧光定量PCR 技术本身的优越性把其 quantitative PCR，RTFQ PCR ）是在常规PCR 基础上把 广泛运用于医学诊断、海关检验检疫、国防军事、新型 [1-2] 荧光共振能量转移与荧光标记探针结合，巧妙地把核 农业、基础研究等领域 。目前尤其在动物医学病理