盐胁迫对绿豆下胚轴氧化还原态影响.pdfVIP

植物生理学报 Plant Physiology Journal 2013, 49 (10): 1 盐胁迫对绿豆下胚轴氧化还原态的影响 1 1,* 1 2 2 顾福根, , , , 赵林川 朱成 吴勇 万华星 1 , 215123; 2 , 215123 苏州大学基础医学与生物科学学院 江苏苏州 苏州科铭生物技术有限公司 江苏苏州 -1 NaCl , GSH/GSSG AsA/DHA NADH/NAD+ : 150 mmol·L 对绿豆下胚轴进行胁迫处理 测定其长度和鲜重以及 、 、 、 摘要 以 NADPH/NADP+ , 48 h 0 h 493.1% 206.9% 661.0% 308.2% 比值。结果表明 对照和处理 时上述指标分别相当于 时的 与 、 与 、 118.1% 72.2% 444.4% 44.4% 96.6% 194.3% 97.1% 154.4% , 与 、 与 、 与 和 与 。可见 盐胁迫处理显著抑制绿豆下胚轴生长可能与 其氧化还原态的显著改变有关。 : 关键词 绿豆; 盐胁迫; 氧化还原态; 谷胱甘肽; 抗坏血酸 Effects of Salt Stress on Redox Status of the Hypocotyls of Vigna radiata L. 1 1,* 1 2 2 GU Fu-Gen , ZHAO Lin-Chuan , ZHU Cheng , WU Yong , WAN Hua-Xing 1School of Biology and Basic Medicine Science, Soochow University, Suzhou, Jiangsu 215123, China; 2Suzhou Comin Biotechnology Co., Ltd., Suzhou, Jiangsu 215123, China Abstract: To investigate the relationship between salt stress and redox status in the hypocotyls of Vigna radiata, the levels of length and fresh weight along with the ratios of reduced and oxidized glutathione (GSH/ GSSG), ascorbic acid (AsA/DHA), nicotinamide adenine dinucleotide (NADH/NAD+) and nicotinamide adenine dinucleotide phosphate (NADPH/NADP+) were determined in the three-old-day seedlings treated with -1 150 mmol·L NaCl for 48 h. Above parameters of control and treatment at 48 h were equal to 493.1% vs 206.9%, 661.0% vs 308.2%, 118.1% vs 72.2%, 444.4% vs 44.4%, 96.6% vs 194.3% and 97.1% vs 154.4% of those at 0 h, re