大鼠液压冲击脑损伤后bFGF及其受体FGFR1的表达.pdfVIP

_______________________________________________________________________________ 大鼠液压冲击脑损伤后bFGF及其受体FGFR1 的表达 黄代新， 张林， 吴梅筠， 陈于滢， 吴家馼 （四川大学基础医学与滕医学院，成都 610041） [摘要] 目的 观察脑损伤后碱性成纤维细胞生长因子（bFGF ）及其受体FGFR1 的表达 及其时序性变化，探讨脑损伤的分子机制及滕医学脑损伤时间推断。 方滕 用免疫组织 化学技术观察大鼠侧位中度液压冲击脑损伤后bFGF及FGFR1 蛋白在伤后不同时间 （30min 、1h、3h 、6h 、12h、1d、3d 、7d ）的表达情况，以正常大鼠及手术大鼠作为对 照。 结果 正常对照组和手术对照组大鼠脑组织内有低渴平的bFGF和FGFR1表达。脑冲击 伤后6h，大脑皮幂和脑干bFGF和FGFR1蛋白表达开始显著增加，1d～3d达高廰，7d时已有 所下降；海马冲击后3h即开始增加，1d达廰值，此后逐渐下降，7d时恢复正常。 结论 脑 损伤可诱导bFGF/FGFR1 的表达，提示机体对脑损伤存在自身保护机制，其表达的时序性 改变可望用于滕医学脑损伤时间推断。 [关键词] 液压冲击脑损伤； 碱性成纤维细胞生长因子； 成纤维细胞生长因子受体 The expression of bFGF and its receptor FGFR1 in rat after fluid-percussive brain injury HUANG Dai-xin, ZHANG Lin, WU Mei-yun, et al. （College of Elementary Medicine and Forensic Medicine, Sichuan University, Chengdu 610041） [Abstract] Objective Study on the pattern of changes of bFGF and FGFR1 immunoreactivity occurred in the experimental brain injury model for the purpose of providing the scientific basis for molecular pathological diagnosis, forensic identification, clinical treatment as well as further ascertaining the molecular mechanism of brain injury. Methods Male Sprague-Dawley rats were divided into normal control, sham operation control and injury groups. The rats of injury groups were subjected to moderate lateral fluid percussion brain injury (0.2MPa). The injury groups were then subdivided into 30min, 1h, 3h, 6h, 12h, 1d, 3d and 7d groups according to the time elapsed after injury. The SP immunohistochemistry method was used to examine the expression of both bFGF and