MTT比色法在抗肿瘤香精油筛选中的应用.pdfVIP

※基础研究 食品科学 2009, Vol. 30, No. 01 75 黄 菲，罗 曼* (暨南大学生命科学技术学院生物医学工程研究所，广东广州 510632) 采用从山苍子油、柠檬草油等香精油分离出的柠檬醛为材料，研究MTT 比色法用于筛选抗肿瘤香精油的 最佳实验条件。比较不同波长选用对吸光度测定的影响；设非挥发性抗肿瘤药物阿霉素为平行实验组，以细胞形 态学变化为依据，分析了不同浓度香精油药物组、对照组在96 孔板上分布对MTT 法测量结果的影响；此外，还 探索药物储藏温度和时间对MTT 法结果重复性的影响。结果表明，选择492 、570 、630nm 三个波长对检测结果 影响甚微；气态香精油物理性位移不仅使得对照组在96 孔培养板上位置直接影响实验结果，而且使得低浓度组(与 高浓度相邻)细胞毒性的MTT 法表征值要高于实际值；挥发性香精油于低温下贮存不仅有益于保持药效，而且能确 保实验重复性。 抗肿瘤香精油；挥发性物理位移；MTT 比色法 Evaluation of Methyl Thiazolyl Tetrazolium (MTT) Assay for Screening Antitumor Essential Oils HUANG Fei ，LUO Man* (Institute of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China) Abstract ：This study focused on citral which is the main component of essential oils such as Litsea cubeba oil and lemongrass oil, and explored a experimental approach of MTT assay in the selection of antitumor essential oils. It compared the effects of different wavelengths on absorbance, taking citral as experimental drug and non-volatile adriamycin (ADM) as control, investigated their volatility and diffusibility according to morphological changes of human K562 cells, and explored the effect of stockage temperature and time on the repetition of MTT assay. It was found that the results followed the same trend at different detection wavelengths as 492 nm, 570 nm and 630 nm. The volatility and diffusibility affect both control and citral treated wells, and make the well that adjoined high concentration well show higher cell toxicity than its actual value. The results also proved the low temperature stockage of volatile essential oils to be not only advisable but also a key point for good experimental repetitions. Key words ：antitumor essential oils