组蛋白磷酸化修饰与精子发生.pdfVIP

Hereditas (Beijing) 2014 3 , 36(3): 220―227 综 述 组蛋白磷酸化修饰与精子发生 1,2 1 1 , , 1. , 071000; 2. , 071000 组蛋白磷酸化是组蛋白氨基酸残基的磷酸化修饰, 是一类重要的翻译后修饰, 与有丝分裂和减数分裂的 染色质压缩、染色质功能调节、转录的激活与抑制、DNA 损伤修复以及物质代谢等多种机制相关。文章对国 内外近 10 年多种代表性生物精子发生(孢子形成) 的相关文献进行总结, 论述了组蛋白磷酸化在精子发生中调 控蛋白质作用因子的结合位点、调控减数分裂过程中的DNA 复制与重组、保障正确的染色质重塑、对减数分 裂后的成熟精子核的完全包装等重要功能。这些发现加深了人们对于组蛋白及其翻译后修饰在精子发生及分化 中作用的理解。 精子质量; 精子发生; 组蛋白磷酸化; 表观遗传 Histone phosphorylation and spermatogenesis Zhaohui Zhang 1,2, Xianjiang Kang 1, Shumei Mu 1 1. College of Life Science, Hebei University, Baoding 071000, China; 2. Department of Reproduction Medicine, Maternal and Babies Health Center of Baoding, Baoding 071000, China Abstract: Histone phosphorylation is one of the most important post-translational modifications (PTMs) which play a key role in the chromatin remodeling process during spermatogenesis. Histone phosphorylation is related to distinct important biological processes, such as mitotic/meiotic chromosome condensation, chromatin function regulation, transcriptional ac- tivation or inactivation, double-strand DNA break repair, and other metabolic reactions. This review discusses the correla- tion between histone phosphorylation and spermatogenesis in different organisms from literatures published in the last dec- ade. The critical functions of histone phosphorylation during spermatogenesis (sporogenesis) are summarized, such as binding sites involved in protein interaction, regulation of meiotic replication and/or recombination, correct chromatin re- modeling, and chromatin compaction in the sperm nucleus (spore) after post-meiotic process. These findings will h