清酒乳杆菌素LSJ618对藤黄微球菌及鸡白痢沙门氏菌的抑菌机理.pdfVIP

中文摘要 乳酸菌细菌素因其高效、无毒副作用且易被人体内蛋白酶降解等优良性质，使 其在食品安全方面具有潜在的应用价值，探讨细菌素的作用机制是更好地发挥其应 用价值的重要理论支撑。本研究初步探讨了清酒乳杆菌素LSJ618 （Sakacin LSJ618 ） 对革兰氏阳性菌藤黄微球菌及革兰氏阴性菌鸡白痢沙门氏菌的作用机理，为清酒乳 杆菌素LSJ618 后续的研究奠定基础。 1、采用二倍稀释法测定了清酒乳杆菌素LSJ618 对藤黄微球菌及鸡白痢沙门氏 菌的抑菌效价（AU/mL ），并测蛋白浓度（mg/mL ），表明清酒乳杆菌素LSJ618 凝 胶层析活性样品对敏感菌的抑菌活力约为209 AU/mg 。 2 、本研究探讨了清酒乳杆菌素LSJ618 对两种敏感菌生长的影响。结果表明： 在初始时刻添加效价为20 AU/mL 的清酒乳杆菌素LSJ618 可在12 h 内抑制藤黄微 球菌和鸡白痢沙门氏菌繁殖；在对数期加入清酒乳杆菌素LSJ618 ，可立即抑制细菌 繁殖，并表现出部分溶菌现象；在一定范围内增加清酒乳杆菌素LSJ618 的浓度可 以增强其抑菌效果。 3、比较清酒乳杆菌素LSJ618 在葡萄糖能量化及非能量化的敏感菌细胞中的抑 菌效果，发现清酒乳杆菌素LSJ618 对能量化的藤黄微球菌抑制作用更好，但在能 量化和非能量化的鸡白痢沙门氏菌间并无明显差异；且葡萄糖的浓度并非越高，效 果越好，100 mmol/L 的葡萄糖足以提高细菌素对敏感菌的抑制能力，高出此浓度无 增强效果。 4 、通过测定敏感菌菌液电导率变化及细胞紫外吸收物质的变化，探讨了清酒乳 杆菌素LSJ618 对敏感菌细胞膜透性的影响。结果表明：清酒乳杆菌素LSJ618 作用 后的藤黄微球菌及鸡白痢沙门氏菌菌液电导率值明显增加，细胞内紫外吸收物质发 生了不同程度的外泄。由此可见，清酒乳杆菌素LSJ618 对敏感菌细胞膜造成了一 定的损伤，致使敏感菌死亡。 关键词：清酒乳杆菌素LSJ618 敏感菌 能量化 抑菌机制 I Abstract Bacteriocins were highly efficient, non-toxic and easy to be degraded. All these natures contributed to that it had a potential application in food safety. Exploring the mechanism of action of the bacteriocin was very important. It was an important theoretical support for food applications . Studies of mode of action of Sakacin LSJ618 against Micrococcus luteus, which were gram-positive, and Salmonella pullorum, which were gram-negative, did a help for further research on this subject. Determinated the activity of Sakacin LSJ618 on Micrococcus luteus and Salmonella pullorum , and the result was 209 AU/mg, by double-dilution method and Lowry which was used to ascertain the protein concentration. It provided an effective guarantee for the in-depth study of Sakacin LSJ618. The test measured the impacts of the growth of the two susc