修饰hTERT核心启动子靶向转录FCY1的重组腺病毒的构建及鉴定.docVIP

修饰hTERT核心启动子靶向转录FCY1的重组腺病毒的构建及鉴定 作者：滑玮，辛晓燕，苏明权，李立文 【关键词】 末端转移酶 　　【Abstract】AIM：To construct and identify transcriptional targeting replication-defective recombinant adenoviruses containing FCY1 driven by hTERT core promoter modified by estrogen response elements and hypoxiaresponsive elements. METHODS: The hTERT core promoter modified by tandem sequences of estrogen response elements and hypoxiaresponsive elements were constructed. The promoter sequence and the FCY1 gene fragment were subcloned into shuttle plasmid, then the shuttle plasmid and rescue plasmid wer