肿瘤相关巨噬细胞通过EMT促进乳腺癌的浸润转移.pdfVIP

网络出版时间：2013-04-24 11:13 网络出版地址：/kcms/detail/31.2035.Q1113.004.html 中国细胞生物学学报 Chinese Journal of Cell Biology 2013, 35(5): http ://www.cj 肿瘤相关巨噬细胞通过EMT促进乳腺癌的浸润转移 宋瑞卉 张宝刚 李洪利 尹崇高 董 惠 郭文君* (潍坊医学院病理学教研室, 潍坊261053) 摘要 探讨肿瘤相关巨噬细胞(tumor-associated macrophages, TAMs)是否通过上皮间质转化 (epithelial-mesenchymal transitions, EMT)促进乳腺癌浸润转移。免疫组化检测80例乳腺浸润性导 管癌中TAMs标记物CD68、E-cadherin和Vimentin 的表达。结果显示, 乳腺浸润性导管癌中CD68阳 性表达率明显高于正常乳腺组织, CD68阳性表达与分化程度、淋巴结转移程度相关, 与E-cadherin 表达呈负相关, 与Vimentin表达呈正相关。分离新鲜肿瘤组织中的TAMs, 细胞免疫荧光鉴定TAMs 分离成功; 乳腺癌MCF-7细胞在TAMs条件培养基培养后: 细胞形态学发生改变; Transwell侵袭实验 表明, 细胞侵袭转移力增强; Western blot结果显示, 细胞中E-cadherin表达明显减少, Vimentin表达明 显增多。由此可得出, TAMs与乳腺癌的浸润转移过程密切相关, TAMs可促进MCF-7细胞发生EMT 进而促进乳腺癌的浸润转移。 关键词 乳腺癌; 肿瘤相关巨噬细胞; 上皮– 间质转化; 浸润转移 Tumor-associated Macrophages Promote Invasion and Metastasis of Breast Cancer by EMT Song Ruihui, Zhang Baogang, Li Hongli, Yin Chonggao, Dong Hui, Guo Wenjun* (Department of Pathology, Weifang Medical College, Weifang 261053, China) Abstract To investigate the mechanism of the tumor-associated macrophages (TAMs) promoting inva- sion and metastasis of breast cancer, TAMs marker CD68, E-cadherin and Vimentin expression were detected in 80 invasive ductal carcinoma specimens by immunohistochemistry. The positive rate of CD68 in invasive ductal carci- nomas was significantly higher than that in normal breast tissue. The expression of CD68 was significantly associ- ated with tumor differentiation and lymph node metastasis and was negatively correlated with E-cadh