小麦卵穗山羊草衍生后代的SSR分子标记鉴定和白粉病抗性评价.pdfVIP

麦类作物学报 2012，32(1)：2227 JournalofTriticeaeCrops Identification ofW heat_AegilopsOvata DerivativesUsingSSR M arkersandEvaluationofTheirPowderyM ildew Resistance WUHong—po，WANGYa-juan，WANGChang-you，JIWan—quan (CollegeofAgronomy，NorthwestA＆FUniversity，Yangling，Shaanxi712100，China) Abstract：AegilopsspeciescarryingtheU or／andM genomesrepresentaprominentsourceofuseful genesforwheatbreeding．W egenerated 19wheat—Aegilopsovata derivativesthrough hybridization betweencommonwheatandAegilopsovata．FourhundredSSR (simplesequencerepeat)markers wereusedtoinvestigatethepolymorphismsamongparentallines，ChineseSpring，Shaanyou225and Aegilopsovata，ofwhich341markers (85．25 )couldamplifybandsinAegilopsovata，and2O of them (5％)showedspecificbandsinAegilopsovata，andwereappliedtoinvestigategermplasm in— heritanceofAegilopsovata inwheat—Aegilopsovataderivatives．Theresultsshowedthat10markers couldamplify specificbandsin the19derivatives，indicating thatallthese 19 derivativesinherited germplasmsfrom Aegilopsovata andthese10SSR markerscouldbeusedin furtheridentification of nextgenerations．W ealsoconductedpowderymildew resistanceevaluationonthe19derivatives，and 16ofthem areimmunetopowderymildew likeAegilopsovata，oneoftheirparents，butnotlikethe othertwoparentallines，ChineseSpringandShaanyou225，which arehighlysusceptibletopowdery mildew，indicatingthatthepowderymildew resistanceinthel6derivativeswereexplicitly inherited from Aegilopsovata． Keywords：W heat—Aegilopsovata derivatives；Powderymildew resistance；SSR markers 小麦一卵穗山羊草衍生后代的SSR分子标记 鉴定和 白粉病抗性评价 吴红坡，王亚娟，王长有，吉万全 (西北农林科技大学农学院，陕西杨凌 712lO0) 摘 要 ：山羊草属植物是普通小麦改 良过程 中重要的有益基 因来源，小麦的许多抗病虫、抗逆基 因都来 源于山羊草属植物 。本研究利用杂交和回交的方法 ，成功获得 了 19株小麦一卵穗 山羊草衍生后代 ，实验选取 均匀分布于小麦各条染色体的SSR标记 400个，对三个亲本(中国春 、卵穗 山羊草和陕优 225)以及