microrna125a质粒表达载体构建及其表达活性.pdfVIP

中国组织工程研究 第 16 卷 第2 期 2012 –01 –08 出版 Chinese Journal of Tissue Engineering Research January 8, 2012 Vol.16, No.2 MicroRNA-125a质粒表达载体的构建及其表达活性★ 张 涛，李 东，李 华，高 辉 Construction and expressing activity of microRNA-125a plasmid expression vectors Zhang Tao, Li Dong, Li Hua, Gao Hui Abstract BACKGROUND: pSuper is a kind of eukaryotic expression vectors of non-coding RNA. It expresses the precursor of miRNA in Center of Tumor plasma, and then the precursor is cut by Dicer enzyme to produce mature miRNA. This process completely imitates the formation Diagnosis and process of endogenous miRNA