游离脂肪酸对型糖尿病大鼠心肌细胞凋亡的影响及机制探讨.docVIP

游离脂肪酸及一氧化氮对2型糖尿病大鼠心肌细胞凋亡的影响 郝拥玲 祁燕 李凯利 （1新疆医科大学附属中医院内分泌科830001） [摘要]目的 以2型糖尿病大鼠为实验对象，观察是否可出现心肌细胞凋亡并探讨其机制。方法 制备2型糖尿病大鼠模型，2月后，处死检测一般指标，并观察心肌细胞凋亡的情况和心肌的病理变化及血中炎症介质、游离脂肪酸（Free Fatty Acid，FFA）和一氧化氮（Nitric Oxide，NO）的表达。结果（1）与正常对照组比较，糖尿病模型组的空腹血糖（FPG）,空腹血清胰岛素（FINS）,稳态模式胰岛素抵抗指数（HomaIR）,甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白（LDL-C）,FFA，NO，肿瘤坏死因子-α（TNF-α）,白细胞介素-6（IL-6）,C反应蛋白（CRP）,血浆纤溶酶原激活物抑制因子（PAI-1）明显增高（P0.01，P0.05），稳态模式胰岛β细胞功能指数（HBCI）、高密度脂蛋白胆固醇（HDL-C）明显降低（P0.01）。（2）造模组心肌细胞凋亡远远高于对照组（P0.01）。（3）镜下可见糖尿病大鼠心肌细胞排列紊乱，心肌细胞减少，可见核固缩。 结论 动物模型出现了明确的心肌细胞凋亡特征性表现,可能与FFA，NO和前炎症介质相互作用，共同促进有关。 [关键词]2型糖尿病；心肌细胞凋亡；游离脂肪酸；一氧化氮 The Effects of Free Fatty Acids and Nitric Oxide on Cardiomyocyte apoptosis in the Type 2 Diab etic Rats Hao Yong Ling, Qi Yan, Li Kai-Li (1Department of Endocrinology, The Traditional Chinese Medicine Affiliated Hospital of XinJiang Medical University,2Department of Endocrinology,First Affiliated Hospital of XinJiang Medical University Urumqi 83000 China) Abstract Objective : To observe whether the cardiomyocyte apoptosis will be occured in the model of the type 2 diabetic rats and explore its mechanism.Method:The model of type 2 diabetic rats were made. After two month, the blood samples were collected to exam . With the help of microscope, we observed how cardiomyocyte apoptosis occurred and examed expression of inflammatory factors, free fatty acid(FFA) and nitric oxide(NO) in the blood.Result（1）FPG,FINS,HomaIR,TC,TG,LDL-C,FFA,NO，TNF-α,IL-6,CRP,PAI-1 of the diabetic rats were significantly increased（P0.01，P0.05）and there was a remarkable decrease of HomaCI and HDL-C in comparison with the normal controls（P0.01，P0.05）.(2)With the help of microscope,the expression of cardiomyocyte apoptosis of the diabetic rats were much more higher than the normal controls （P0.01）appeared the typical charectrestics of cardiomyocyte apoptosis.Its mechanism is possibly associated with the interactions of inflammation factors,FFA and NO. [Key Words]:Type 2 diabetes；cardiomyocyte apoptosis；Tumornecr