Beinhart1000_1抗赤星病基因的QTL定位_高亭亭.pdfVIP

104 中国烟草学报　2014 年4 月 第20 卷 第2 期 Beinhart 1000- 1 抗赤星病基因的QTL 定位 ， ， ， ， ， 高亭亭 蒋彩虹 罗成刚 杨爱国 程立锐 代帅帅 烟草行业烟草基因资源利用重点实验室，中国农业科学院烟草研究所，青岛266 10 1 摘　要：为探索Beinhart 1000-1 的赤星病抗性遗传规律，以抗赤星病品种Beinhart 1000-1 为母本，感病品种G140 为父本，构建 F1 及F2 代群体，筛选与抗性基因紧密连锁的SSR 标记，并进行抗性基因的QTL 定位。结果表明，Beinhart 1000-1 的赤星病抗性 由显性基因控制。同时利用混合群体分组分析法，从2653 对SSR 引物中，得到83 对在抗感池间表现多态且扩增条带稳定清晰 的SSR 标记。以F2 代群体115 个单株为作图群体，利用该83 对引物进行扩增，经WinQTLCart2.5 分析，构建了83 个标记的遗 传连锁图谱，获得2 个抗赤星病QTL 位点，分别位于7 号和15 号连锁群上。 关键词：烟草; Beinhart1000-1 ；赤星病; SSR; QTL 定位 doi:10.3969/j .issn.1004-5708.2014.02.018 中图分类号:S47; Q78　　　文献标志码：A　　　文章编号：1004-5708(2014)02-0104-04 Mapping of quantitative trait loci affecting resistance to brown spot in tobacco line Beinhart1000-1 GA O Tingting, J IAN G Caihong, LU O Chenggang, YAN G Aigu o, CHEN G Lirui, DAI Shuaishuai Key Lab oratory for Tob acco Gene Resources, Tob acco Research In stitute, Chinese Academy of Agricultural Sciences, Qingdao 266 10 1, Shandong ，China Abstract: F1 and F2 populations were constructed from a cross between Beinhart 1000- 1 and susceptible variety G 140 to study the genetic basis of resistance to brown spot in tobacco cigar line, Beinhart 1000- 1, and to identify genomic locations contributing to resistance. Inheritance of resistance to brown spot was analyzed in F1 and F2 populations. Results shown that resistance to brown spot in Beinhart 1000-1 was controlled by dominance genes. A population of 115 F2 was further used for QTL mapping with 83 SSR markers. Two QTLs, RBS-1 and RBS-2, were discovered on No.7 and 15 genetic linkage groups through composite interval mapping method. The maj or QTL, RSB-1, was flanked by marker M935 with ge