miR396在豆科植物苜蓿根中对其菌根化和分生组织活性影响.pptVIP

* * miR396a启动子首先在根瘤导管组织中发现，miR396b启动子在维管组织，感染区（Ⅱ）和氮固定区（Ⅲ）含量很高 * * 虽然miR396存在于根瘤中，但miR396-OE和MIM396对根瘤密度都没有明显影响 We also analysed the expression of miR396 in response to Myc-LCOs, the lipochitooligosaccharide signal molecules produced by AM fungi. The involvement of miR396 during mycorrhization was then analysed at 5 weeks after colonization with R. irregularis (formerly Glomus intraradices) in miR396-OE and MIM396 roots. * * * * miR396 affects fungal colonization during AM symbiosis. 菌根的丛枝量和形态没变化，但AM定植在miR396-OE根中的量减少，而在MIM396中的量增加 Hence, the action of miR396 limits mycorrhizal colonization. Here, we showed that, in the model legume M. truncatula, miR396 represses not only MtGRF but also two MtbHLH79 TF-encoding genes, and that its de-regulation affects root growth and the ability to be colonized by mycorrhizal fungi. * * * * * Institute of Computer Software Nanjing University Institute of Computer Software Nanjing University * * miR396 affects mycorrhization and root meristem activity inthe legume Medicago truncatula The Plant Journal (2013) doi: 10.1111/tpj.12178 * * 主要内容 INTRODUCTION RESULTS 1、 Mtr-miR396a and mtr-miR396b genes show different tissue-specific expression profiles in Medicago truncatula roots 2、In M. truncatula roots, miR396 regulates six GRF and two bHLH79 transcription factors 3、Characterization of the miR396 regulatory network in M. truncatula 4、 miR396 negatively affects root growth, RAM size and cell proliferation in the root apex 5、 Silencing of MtGRF genes mimics the miR396-OE root growth phenotype 6、 Hormonal control of miR396 expression 7、miR396 limits mycorrhizal colonization but not nodulation * * INTRODUCTION In plants, root growth is controlled via coordinated cell division and expansion at the root apex, where three regions are observed: (i) the meristematic zone, (ii) the elongation zone, and (iii) the differentiation zone Root development is regulated by complex interactions of hormone signalling pathwa