云南白族、傣族、彝族X染色体STR基因座的遗传多态性及其与中国5个主要民族的遗传关系.pdfVIP

HEREDITAS (Beijing) 2009 2 , 31(2): 153―159 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2009.00153 云南白族、傣族、彝族X 染色体STR 基因座的遗传多 态性及其与中国5 个主要民族的遗传关系 魏曙光, 杨丽, 郑海波, 沈靓, 赖江华 , , 710061 应用复合 PCR 及基因扫描技术, 对云南白族、傣族、彝族人群 X 染色体 3 个 STR 基因座 DXS6804 、 DXS6799 、DXS7132 的遗传多态性进行研究。白族89 个样本中共检出 18 个等位基因, 38 个基因型, 等位基因 频率分布在0.0200~0.6400 之间, 基因型频率分布在 0.0256~0.3333 之间; 傣族 100 个样本中共检出 17 个等位 基因, 24 个基因型, 等位基因频率分布在0.0135~0.7500 之间, 基因型频率分布在0.0385~0.5769 之间; 彝族88 个样本中共检出 20 个等位基因, 35 个基因型, 等位基因频率分布在 0.0125~0.5875 之间, 基因型频率分布在 0.0250~0.3500 之间。群体遗传多态性指标及法医学应用指标统计结果显示, 3 个基因座在云南3 个少数民族人 群中均具有高度多态性。聚类分析和系统进化关系分析发现, 彝族、白族、傣族与藏族之间的遗传关系较近。 少数民族; 短串联重复序列; 遗传多态性 Genetic structure of X-STR loci in Bai, Dai and Yi ethnic groups and their affinity with five major populations of China WEI Shu-Guang, YANG Li, ZHENG Hai-Bo, SHEN Liang, LAI Jiang-Hua State Key Subject for Forensic Sciences, the Key Laboratory of National Ministry of Health for Forensic Sciences, College of Medicine, Xi’an Jiaotong University , Xi’an 710061, China Abstract: To determine the genetic polymorphism of three X-STR loci for Bai, Dai, Yi ethnic groups from Yunnan Prov- ince, DXS6804, DXS6799 and DXS7132 were genotyped by multiplex PCR and Genscan. Eighteen alleles and thirty-eight genotypes were detected in 89 Bai unrelated persons. The gene frequencies ranged from 0.0200 to 0.6400, and the geno- types frequencies ranged from 0.0256 to 0.3333. Seventeen alleles and twenty-four genotypes were detected in 100 Dai unrelated persons, with the gene frequencies ranging from 0.0135 to 0.7500 and the genotypes frequencies ranging from 0.0385 to 0.5769 respectively. There were 20 alle