冻干前人红细胞对海藻糖的负载影响因素研究.pdfVIP

冻干前人红细胞对海藻糖的负载影响因素研究 刘景汉陈麟风欧阳锡林庄远车辑 解放军总医院输血科全军临床输血中心 北京100853 【摘要】目的研究影响海藻糖负载多种因素，根据红细胞海藻糖的负载量衡量，探讨红细胞负载海藻 糖的规律性。方法利用硫酸．葸酮法检测红细胞在不同胞外海藻糖浓度、不同孵育时间、不同孵育温度 的条件下对海藻糖的摄取量并检测红细胞溶血程度。结果红细胞内海藻糖的量和负载液中海藻糖浓 度、负载时间、负载温度呈正相关。对温度、时间和胞外海藻糖浓度的统计分析显示温度对负载后胞内 载缓冲液中孵育7ll能有效摄取海藻糖，使之达到理论保护浓度，为红细胞的长期冻干保存奠定了基础。 【关键词】海藻糖负载、负载缓冲液、冻干红细胞 factorsinfluencethetrehalose rluefor The that the RBCs谢tll IAbstractlObject loading，discussloading lrehaloseacrosstotheinuacellul缸trehalose．MethodsRBCsweredissolvedmdifferentconditionsof was to incubation andextracellulartrehalose reactionused temperature，duration concentration，anthrone determinetheamountoftheintracellular weevaluateextracellularfree of trehalose，meanwhile hemoglobin loadedRBCs．ResultstrehaloseinRBCahadclosedcorrelationwiththe and loading temperature，duration extracellulartrehaloseconcentration．Forthese statisticalresultsshowedthatthe had factors，the temperature The themosteffctsonintracellulartrehalose．Conclusionmethod efficientofthetrehaloseis provides uptake freshRBCswhichwere in800mmol／LWhaloseat37Cforatime of ina choosing incubated span7h，resulting efficientintracellularlrehaloseconcen仃ationsofabout52mmol／L．This an fwst in importantstep studyprovides ofRBCs． long—termpreservation Words】trehalose redbloodcells IKey loading；loadingbuffer；lyophilized 内源性的或是外源性的海藻糖对生物体和生物大分子都