水稻花色素苷合成调节基因hrd1_t_鉴定_刘永柱_罗文龙_黄翠红_陈立凯_王.pdfVIP

2013,46(19):3955-3964 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.19.001 hrd1(t) /510642 Characterization of the Regulatory Gene hrd1(t) Involved in Anthocyanin Biosynthesis LIU Yong-zhu, LUO Wen-long, HUANG Cui-hong, CHEN Li-kai, WANG Hui, CHEN Zhi-qiang, GUO Tao (South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642) Abstract: Objective The objective of this study is to conduct genetic analysis and gene mapping for the purple leaf character of rice germplasm hrd1 (Oryza sativa L. ssp. indica). Method The regulatory gene hrd1, is a rice germplasm with purple leaf which originated from the hybrids of two green leaf accessions Texianzhan 13 and 02428. Major agronomic traits of hrd1 was firstly investigated, and the segregation ratio of purple and green leaf characters was assayed by using the F , F and BC F 1 2 1 1 populations derived from the crosses of hrd1 and 02428. Fine mapping for the gene contributed to purple leaf character was further conducted by using F2 population, and the candidate gene was identified. Result The purple character initiates on leaf apex of hrd1 at the 2nd leaf stage and gradually spreads to most parts of the whole plant. At the ripening stage, the agronomic trait of hrd1 was significantly different from the control cultivar (Texianzhan 13). Chlorophyll content was equal between hrd1 and the control, while the anthocyanin content in hrd1 was much higher than that in control. A recessive gene named hrd1(t) controls the purple leaf char