兰属Cymbidium植物ISSR遗传多样性分析.pdfVIP

HEREDITAS (Beijing) 2008 年5 月, 30(5): 627―632 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.00627 兰属Cymbidium 植物ISSR 遗传多样性分析 1 1 1 2 吴振兴 , 王慧中 , 施农农 , 赵艳 1. 杭州师范大学生物化学与分子生物学杭州市重点实验室, 杭州 310018; 2. 浙江工商大学食品、生物与环境工程学院, 杭州 310018 摘要: 将简单重复序列区间扩增多态标记(inter-simple sequence repeats, ISSR)技术应用于 16 种兰属的遗传多样 性分析, 15 个引物共扩增出836 条带, 其中有227 条多态带, 多态百分比为27.2% 。UPGMA 聚类结果显示: 春 兰与春剑的亲缘关系最近, 而兔耳兰与其他 15 种兰属植物的距离最远。ISSR 聚类结果与传统分类结果基本相 似, 表明该技术能在分子水平上对传统兰属分类进行必要补充。 关键词: 兰属植物; 遗传多样性; ISSR The genetic diversity of Cymbidium by ISSR 1 1 1 2 WU Zhen-Xing , WANG Hui-Zhong , SHI Nong-Nong , ZHAO Yan 1.Key Laboratory of Biochemistry and Molecular Biology, Hangzhou Normal University, Hangzhou 310018,China; 2. College of Food, Biological and Environmental Engineering, Zhejiang Gongshang University, Hangzhou 310018, China Abstract: ISSR was applied to detect the relationship between 16 Cymbidium species, and 836 bands were amplified with 15 primers, including 227 polymorphic bands. The polymorphic percentage is 27.2%. UPGMA results showed that the ge- netic distance were closest between C.goeringii (Rchb.f.) Rchb.f. and C. goeringii var. longibracteatum, and C.lancifolium Hook. was far away from the other 15 species. This result is quite similar to the traditional classification, indicating that the technique could supplement some information to traditional taxonomy in the molecular level. Keywords: Cymbidium; polymorphism; ISSR 兰属是兰科兰族的萼足兰涯族的一个属, 全世 兰属植物在自然界中存在杂交, 也有人通过属 界约有兰属植物 70 种[1], 主要分布于亚洲热带与亚 间杂交培育出优良品种, 因此该属的分类较为混 热带地区, 向南到澳大利亚北部。根据 Du