黑色素瘤个体化诊治与思考.pdfVIP

316 药 物 生 物 技 术 第21卷第4期 (3)：206． (1O)：1544． [11]ZhangJ，LiHX，WangXY，eta1．Phage—DerivedFuHyHuman [13]杨军，陈明清，董坚．VEGF／VEGFR-2信号转导通路在抗肿瘤 AntibodyscFvFragmentDirectedAgainstHumanVascularEndo— 血管生 成 中的作 用 [J]．世界华人 消化 杂志，2007，15 thelialGrowth FactorReceptor2 Blocked Its Interaction with (34)：3611． VEGF[J]．BiotechnolProg，2012，28(4)：981． [14]YoussoufianH，HicklinDJ，RowinskyEK．Review：Monoclonal [12]李致科，何远，张娟，等．抗VEGFR-2全人源 IgG1抗体的构建 AntibodiestotheVasculra EndothelialGrowth FactorReceptor-2 及其 在 CHO-k细胞 中的表 达 [J]．药学 学报，2013，48 inCnacerTherapy[J]．ClinCancerRes，2007(13)：5544． StudyontheAnti-M elanomaActivitiesofFullyHumanAntibodyagainst VEGFR．2in Vitroandin Vivo LIDao-juan，ZHANGJuan ，JIAXue—lian，FANGJing—ru，WANGMin (SchoolofLfieScienceandTechnology，ChinaPharmaceuticalUniversity，Nanjing210009，China) Abstract Vascularendothelialgrowthfactor(VEGF)，highlyexpressedinmosttumorcells，isavitalmediatorofangiogenesis，and VEGFreceptor2(VEGFR2)isoneofitsspecificmembranereceptors．Researchershavefoundthatblockingthesignalpathwayof VEGF／VEGFR2canrestraintheangiogenesisprocessesandinhibitthesurvival，proliferation，mirgationoftumorcells．Toinvestigate theanti—tumoractivityofthefullyhumanantibody (Mab：04)againstVEGFR-2onmelanomabothinvitroandinvivo，Flow eytometrywasappliedtodetectthecombinationrateofMab一04withmelanomacells；MTFassaywasutilizedtodetecttheeffectof Mab一04ontheproliferationofmelanomacellsinvitro：scratch—woundhealingassayandAnnexinV／PIdouble—stainingassaywere appliedtodetecttheinfluenceofMab一04 onmirgation ofmelnaomacellsandtheapoptosisinducedbyMab-40 ．Additionally，a B16F10一bearingmousemodelwasestablished，andH．E．(hematoxylin—eosin)stainingexaminationwasutilizedtodetecttheanti— tumoreffectsofMab-40 invivo．Theanti—tumorresultsinvitroshowedthatMba 一40 couldspecificallycombinewithB16F1