45种放线菌发酵液对香蕉枯萎病菌的室内毒力测定.pdfVIP

广东农业科学 2008 年第 11 期 65 45 放线菌发酵液对香蕉枯萎病菌 的室内毒力测定 庆菊1 2 1 1 2 曾会才 何 娜 1援中国热带农业科学院热带生物技术研究所袁海南海口 571101曰 2援海南大学环境与植物保护学院袁海南儋州 571737冤 摘要 从五指山热带原始林区的土壤 分离获到45 株对香蕉枯萎病具有拮抗作用的放线菌袁在对放线菌进行发酵 后用旋转蒸发仪浓缩10 倍袁并制成带毒的培养基后袁培养香蕉枯萎病菌6 d遥 试验结果表明袁45 株放线菌的发酵液对香蕉 枯萎病菌具有不同程度的抑菌作用袁其中代号为 162尧378尧217尧38 等4 菌株的抑菌效果明显遥 关键词 香蕉镰刀菌枯萎病曰 拮抗菌曰 毒力测定 + 中图分类号 S476 .1 文献标识码 B 文章编号 1004-874X 2008冤11-0065-02 Toxicity measurement of 45 actinomycosis strains Fermentation solution on Fusarium oxysporum 1袁2 1 1袁2 WU Qing-ju 袁 ZENG Hui-cai 袁 HE Na 1.State Key Biotec hnology Laboratory for Tropical Corps 袁CA TA S,Haikou 571101 袁 China曰 2.college of env ironme nt and Plant Protec tion 袁Hainan Uninv ers ity 袁Danzhou 571737袁 China冤 Abstract 院To be separated 45 the antagonism actinomycosis Strains of banana vascular wilt disease from the soil of the WUZhi-shan tropical primitive forest areas .The actinomycosis strains fermented used rotary Evaporation 10-fold enrichment.The concentrated solution used to be Made from infected medium. Banana wilt Pathogens Fungi reared in the Toxicity culture medium.after the six days of inhibition was observed. The results showed that the 45 actinomycosis Strains fermentation solution had different degrees of inhibition effect on the banana vascular wilt. 162,378,217,38,the four actinomycosis Strains had more obviously inhibitory effect.