双项同测法测定血清丙氨酸转氨酶和天门冬氨酸转氨酶.pdfVIP

2002,27( 2) 146 BULL HUNAN MED UNIV 蒋洪敏 ( 中南大学湘雅二医院检验科, 长沙 410011) [ ] 目的: 研究一种可以同时测定血清丙氨酸转氨酶( ALT) 和天门冬氨酸转氨酶(AST) 的方法方法:用自 配的 项同测试剂同时测定血清中的ALT 和AST 结果: 项同测试剂测定ALT 和AST 与成品试剂比较, 相关性好 ( 均 = 0. 999) ; 精密度高,批内变异均低于5% ; 抗干扰能力强, 溶血黄疸脂血不影响结果; 检测速度较传统方法快 r 一倍结论: 项同测法既能充分发挥仪器的功能又提高了测定速度, 是一种值得推广的方法 [ ] 丙氨酸转氨酶; 天门冬氨酸转氨酶; 项同测法* + [ ] R46611 2 [ ] A [ ] 2002) Twin test in the determination of alanine aminotransferase and aspartate aminotransferase in the serum JIANG Ho gmi ( Dep artment of Clinical Laboratory , Second X iangy a H osp ital , Central South University , Changsha 410011, China ) Abstract : Objective To explore a method which ca simulta eously determi e ala i e ami otra sferase (ALT) a d aspartate ami otra sferase ( AST) i the serum. Methods Usi g the selfprepared twi test re age t, ALT a d AST i the serum were detected simulta eously. Results There was a good correlatio betwee the twi test s method a d the traditio al method i the detectio of ALT a d AST ( All r = 0. 999) . The preci sio of the method was good, a d the coefficie t variatio ( CV) was below 5% . Hemolysis, jau dice, a d lipo hemia serum were fou d to be i terfere cefree. The time of the detectio was two times of the traditio al meth od. Conclusion The twi test method ca both exert the capability of the automatic a alyzer a d shorte the time of detectio . It is a method worthy to be