实验性肝细胞癌SPIO增强MRI及其病理基础研究.docVIP

实验性肝细胞癌SPIO增强MRI及其病理对照研究 廖翠薇(((，邹利光，卫 静，刘卫金，梁开运（重庆市第三军医大学新桥医院放射科 重庆 400037） （本教研室原创，发表于：第三军医大学学报，2006年第一期。wjl上传） 提 要：目的 探讨实验性肝细胞癌超顺磁性氧化铁（superparamagnetic iron oxide，SPIO）微粒增强磁共振成像（magnetic resonance imaging, MRI）表现及其病理基础，以期通过SPIO增强表现推测肝细胞癌的病理分级。方法 (1)健康清洁级雄性Wistar大鼠90只，随机分为实验组80和对照组10，实验组灌喂2‰二乙基亚硝胺(diethylnitrosamine, DEN)生理盐水溶液。 (2)大鼠分别于灌药后第16、18、20、21、22、23、24、25周行MRI扫描，先行SE-T1WI、FMPSPGR-T1WI、GRE-T2WI和FSE-PDWI、FSE-T2WI的MRI平扫检查，再采用相同序列和参数进行SPIO增强MRI检查，并选取兴趣区获取相关参数。(3)扫描结束后，处死大鼠，观察肿瘤的大体形态，并按照MR扫描层面对应部位取材，HE染色和PerIs染色。结果 (1)肝细胞癌的Kupffer细胞数明显低于正常肝组织(P﹤0.01)，肝细胞癌的Kupffer细胞数与病理分级相关。(2)肝细胞癌的SIRSPIO与病理分级及Kupffer细胞数相关。结论 肝细胞癌中Kupffer细胞的含量少于正常肝组织，随着分化程度的降低Kupffer细胞减少或缺乏，而SIRSPIO增高。 关键词：超顺磁性氧化铁；磁共振成像；肝细胞癌；病理分级；Kupffer细胞；序列；大鼠 Study on SPIO-enhanced MRI of HCC with pathology in rats LIAO Cui-wei, ZOU Li-guang, WEI Jing, LIU Wei-jin，LIANG Kai-yun(Department of Radiology,Xinqiao Hospital, Third Military University, Chongqing 400037, China) –enhanced MRI in rats, so as to clarify the pathologic basis of SPIO-enhanced MRI that is useful for estimation of histological grading in HCC. Methods 1. Ninety male rats of Wistar were divided into two groups randomly, 80 were test group and 10 were control group. Diethylnitrosamine(DEN) was dissolved in saline at a concentration of 2? 2. The hrats received MR examinations in 16, 18, 20, 21, 22, 23, 24, 25 week. MR imaging was performed before and after SPIO injection using SE-T1WI, FMPSPGR-T1WI, GRE-T2WI, FSE-PDWI, FSE-T2WI, and the relative parameters were obtained. 3. Rats were killed as soon as MRI finished, to match with the lesions seen on the MRI, tumors were observed grossly and drew for pathologic examination. All specimens were stained with HE and PerIs. Results 1. Kupffer cells numbers in HCCs were significantly smaller than in liver tissues(P＜0.01), and were correlated with the histological grading. 2. The SIRSPIO of HCC was correlated with the histological grading and the Kupffer-cell-count ratio. Conclusion The number of Ku