47种中草药提取物的体外抗菌活性筛选研究.pdfVIP

维普资讯 · 中药视窗 · 47种中草药提取物的体外抗菌活性筛选研究 左国营 ，韩 峻，余 巍 ，徐贵丽，王根春 (1．成都军区昆明 医院药剂科，昆明市 650032；2．云南中医 学院，昆明市 650031) 中图分类号 R285 文献标识码 A 文章编号 1001—0408(2005)10—0798—02 摘 要 目的：测定47种中草药的80％乙醇提取物的体外抗茵活性。方法：对大肠埃希茵、铜绿假单胞茵进行抗生素药敏试验；制 备47种中草药醇提物，采用常规琼脂扩散法进行体外抑茵试验。结果：47种中草药的醇提物中有 11种对大肠埃希茵、铜绿假单胞 茵和白色念珠茵有不同程度的抑制活性；其中尤以赤芍和石榴皮的醇提物抗耐药茵活性最高，赤芍醇提物对上述 3种茵特别是耐 药茵的抑制活性均较高，其最低抑茵浓度 (MIC)分别为 1．3、1．1、1．8mg／m1；石榴皮醇提物对上述 3种茵的抑制活性也较高，其 MIC分别为8．3、3．6、2．3mg／ml。结论：赤芍和石榴皮醇提物呈现广谱抗茵特性，且具有抗耐药茵作用。 关键词 中草药；醇提物；抗茵活性；最低抑茵浓度 AntimicrobialActivitiesScreeningof47TraditionalChineseHerbalMedicineExtracts ZUOGuoying，YUWei，XU Guili，WANGGenchun(Dept．ofPharmacy，KunmingGeneralHospitalof ChengduMilitaryRegion，PLA，Kunming650032，China) HANJun(YunnanTCM College，Kunming，650031，China) ABSTRACT OBJECTIVE：Todeterminetheantimicrobialactivityofalcoholicextractsof47traditionalChineseherbal medicinesinvitro．METHODS：AntibioticdrugsensitivetestsofEscherichiacoliandBacilluspyocyaneuswereconducted．al— coholicextractsof47Chineseherbalmedicineswereprepared，whichweresubiectedtobicteriostatictestsinvitrobyagar spreadroutinemethod．RESULTS：Amongthe47traditonalChineseherbalmedicinesalcoholicextracts．11ofwhichdidhave antimicrobialactivityagainstescherichiacoli，pseudomonasaeruginosaandblastomycesalbicansindifferentdegree；among which，theantimicrobialactivityofthealcoholicextractsofpaeoniaeradixandgranatum todrugresistancebacteriawerethe highest，thealcoholicextractsofpaeoniaeradixshowedstronginhibitoryactivitiestotheabove3bacterium，especiallytothe resistantorganismswiththeminimum inhibitoryconcentrations(MIC)at1．3，1．1and1．8mg／mlrespectively；Theantimi— crobialactivitiesofalchoholicextractsofgranatum againsttheabove3bacterium werealsohighwiththeM ICat8．3，3．6and 2．3mg／mlrespectively．C0NCLUS10N ：Thealcoholicextractsofpaeoniaeradixandgranatum havebroadspectrum ofan— timicrobialactivityandwhichhaveantimicrobialeffecttodrugresistancebacteria． KEY WORDS TraditionalChineseherbalmedicine；Alcoholicextracts；An