长白蝮蛇类凝血酶基因(Ussurase)克隆及分析.pdfVIP

维普资讯 嚣拳螽，期 J。uRNALoFHA海IN南AN师N范OR学MA院L学U报NIVE自‘R然SI科TY学(N版AT’URALScIENcE) ． OoningandanalysisofeDNA forthrombin-likeeazylm ， DUI~-lin1，DENGⅪa ，wuWen-fang，LUC-,．tlo-an1，XU Mei1 (1．InstituteofAppliedEcology，AcademiaSiniea，Shenyang110016 2．DepartmentofBiology，HainanNormalUniversity．Hmkou571158； 3．NationalKeyBiotechnologyLaboratoryforTropicalCrops．CATA，Haikeu571101，China) Abstract：TotalRNA was extracted from thevenom gland oftheAgklstrodon halysUssuriensiasnake．Tl1e thrombin— l eenzymegenewasamplified by RT — PcR，doned。anditsnucleotidesequencehadbeendetermined．The~ngyolecalled us． surased)NA encodes699 nucleotides．namely 233 Rmino acids．Based on hte bo ． mology。thecatalyticresidues anddisulfidebridges ofu~surasewerededucedasfollows： catalyticresidues．H 。，AspaandSe~’’；anddisulfidebridg∞，cys7一Cys 。Cys2 一 Cys4，cy 一cys2，Cyst’一Cy； ，Cys1蚺一Cys64andCys圩一cys产∞． Keywords：AgkistrodonhalysUssuriem is；thrombin—llkeenzymegene；ussur· ase；elone；sequenceanalysis SDa~evenom affectsbloodcoagulation andplateletfunctionsinSocmplex manner[¨． Th e v朗啪 ofht esnekeAgkistrodonhalysUssuriensiscontainsbo ht platdet—activatingna dplatelet — inhibitoryocmponents．ahaemorrhagicproteinna dathmmbin—likeenzyrae【 】 ． Thrombni— likeen~ftneisa~rineprotease[3-s] which belongstO thetrypsinfamily【 。 ． In ocntrastwith thrombin，whichocnverts fibrinogeninto fibrni bysplitting offfibrlnopeptideA andB．mostof thrombni-likeenzylnesplitsofffibrinopeptideAf- ． ParenterM administrationofhteenzyme ℃枷 船 theocnversionoffibr