关键字有机铝复合物,铝分布,电子显微镜,X射线微量分析,冷冻乾燥.docVIP

茶樹免除鋁的營養生理障礙之研究 梁致遠1* 顏江河2 林鴻淇1 1國立台灣大學農業化學研究所 2台灣省林業試驗所 摘要 木質部汁液是輸送金屬化合物的介質，收集茶樹莖部的木質部汁液，以離子層析儀分析木質部汁液中的鋁物種。且以不同比例的含鋁水耕液栽培茶樹，採掫茶樹成熟葉片，以組織化學方法、冷凍乾燥、電子顯微鏡X射線微量分析法討論葉片中金屬元素的微量分佈及葉片中鋁的組成成分。結果顯示，茶樹木質部汁液的pH是4.7，其中有機鋁複合物是一重要的鋁物種，而鋁離子僅佔小部分。茶樹成熟葉片的含鋁量超過2,000μg/g。茶樹吸收的鋁，經木質部運送至蒸散流末端累積起來，累積的部位主要是茶樹葉片的上、下表皮細胞壁上。在高含量鋁的培養液中，茶樹成熟葉片之表皮細胞壁的鋁含量遠大於鈣，但低含量的鋁培養液中則相反，表皮細胞壁上的鋁含量低於鈣，顯示在表皮細胞壁上的鋁能和鈣競爭細胞壁的官能團，進而在表皮細胞壁上堆積。 關鍵字:有機鋁複合物，鋁分佈，電子顯微鏡，X射線微量分析，冷凍乾燥 Avoidance Mechanisms of Physiological Interference of Aluminum in Tea Plant Ji-Yuan Liang1*, Chiang-Her Yen2 and Hong-Chi Lin1 1 Graduate Institute of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, R.O.C. 2 Taiwan Forest Research Institute, Taipei, Taiwan, R.O.C. Abstract The fluid xylem is a medium for forming and transporting metal complexes. Tea plants (Camellia sinensis L. cv. T.T.E. No.12) were cultured in an extreme acid hydroponic solution to study the aluminum complexes of xylem sap by ion chromatography and the distribution of aluminum in the mature leaf by of tea plant by X-ray microanalysis using an electron microscope and a freeze drying method. The results showed that the pH of xylem sap was 4.7, that the organo-aluminum complex was significant among the aluminum species, and the aluminum ion was insignificant in the xylem sap. The content of aluminum in the mature leaf of the tea plant was more than 2,000μg/g. Aluminum accumulates densely accumulated in the cell wall of the epidermis.The content of aluminum in the cell wall of epidermis of mature leaves was greater than that of calcium for the high aluminum hydroponic solution, but the opposite results were found the low alumin