锦灯笼抗炎药效物质基础及其作用机制.pdfVIP

锦灯笼抗炎药效物质基础及其作用机制 陈雷 1 何玉静 1 宁立华 1 庞敏 1 邱峰 2 赵烽 1 1 烟台大学药学院 264005 烟台 2 沈阳药科大学中药学院 110016 沈阳 摘 要：目的：研究锦灯笼中的活性成分酸浆苦素 A 与木犀草素、木犀草苷的协 同抗炎作用。方法：用 LPS 刺激小鼠单核巨噬细胞 RAW 264. 7 释放 TNF-α、NO 等炎症介质，评价药物对巨噬细胞释放炎症介质的抑制作用。ELISA 法检测 TNF-α的含量、Griess 法检测 NO 的含量，同时以MTT 法评价细胞毒性。Western blot 法检测 iNOS 及内参蛋白 β-actin 水平。结果：酸浆苦素 A 与木犀草素、木犀 草苷联合用药抑制 LPS 诱导 RAW 264. 7 释放 TNF-α、NO ，有明显的协同作用。 Western blot 结果表明酸浆苦素 A 与木犀草素、木犀草苷联合用药能够显著抑制 iNOS 蛋白水平。结论：酸浆苦素 A 与木犀草素、木犀草苷联合用药具有协同作 用，可以为锦灯笼及相关制剂的临床应用提供一定的理论依据。 关键词：酸浆苦素 A ；木犀草素；木犀草苷；协同抗炎作用 Abstract: Objective: To study the synergistic anti-inflammatory effects of physalin A and luteolin or luteoloside. Methods: Macrophages RAW 264.7 was induced by LPS to release TNF-α and NO, ELISA was used to detect TNF-α content, and Griess assay was used to test NO, while cytotoxicity was tested by MTT method. Western blot was used for detection of iNOS and β-actin protein expression. Results: Physalin A exerted synergistic anti-inflammatory effects with luteolin or luteoloside. Western blot results showed that combination of physalin A and luteolin, physalin A and luteoloside can significantly inhibit iNOS protein expression. Conclusion: The synergistic anti-inflammatory effects of Physalin A and luteolin (or luteoloside) may provide theoretical basis of related preparations for their clinical application. Keywords: physalin A; luteolin; luteoloside; synergistic anti-inflammatory effects 锦灯笼又名挂金灯、红姑娘，为茄科植物酸浆（Physalis alkekengi L. var. [1] [1] franchetii (Mast.) Makino ）的干燥宿萼或带果实的宿萼 。味酸、苦，性寒 。生 于田野、路边及宅旁，产于长白山区各县，多为人工栽培，分布于全国各省区。 该药具有清热解毒、利咽化痰、利尿等作用，用于咽痛、音哑、痰热咳嗽、小便 [2] [3-4] 不利；外治天疱疮、