阴沟肠杆菌(Enterobacter closcae) 对乐果降解特性研究.docVIP

阴沟肠杆菌（Enterobacter closcae）对乐果降解特性的研究( 林抗美1 官雪芳2 鲁国东3 王宗华3 张秋芳1 谢恩3 马丽娜1 刘波1* （1 福州350003 2 福建省农科院农业工程技术研究所,福建 福州350003 3 福建农林大学生命科学学院,福建 福州 350002） 摘要: 从福建省福农生化有限公司排污口的土壤中筛选到一株对乐果有较好降解效果的菌株阴沟肠杆菌,为了使该菌能更好的应用于乐果残留污染的治理当中,特对该菌降解乐果的作用因素进行研究,结果为：菌降解乐果的最佳温度为35℃、pH为8.0、装液量50ml/250ml、摇床振数180r /min、C源为果糖、N源为酵母浸膏、接种量OD265为0.065、底物浓度为200mg/L，对应的降解率28.84%-61.19%之间，采用L18（2*37）正交法及DPS数理统计得出Cu2+，Fe2+对菌降解乐果有的促进Cd2+、Z2+则在溶液中含量低水平时促进降解，高水平时抑制降解；Mg2+、Ba2+、Mn2+、Pb2+对降解。 关键词：阴沟肠杆菌 乐果 降解 Degradation characteristics of dimethoate by Enterobacter closcae LIN Kangmei1,GUAN Xuefang2,LU Guodong3, WANG Zonghua3,ZHANG Qiufang1,XIE En3, MA Lina1, LIU Bo2* (1Agricultural Biological Resources Research Institute，Fujian Academy of Agricultural Sciences，Fuzhou，350003, China),(2 Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Sciences，Fuzhou，350003, China),（3 Life Science College, Fujian Agriculture and Forestry University, Fuzhou 350002, China) Abstrat: By using selective cultural media, Enterobacter closcae was isolated to degrade dimethoate from the soil around the pesticide factory. The factors affecting the degradation of dimethoate by E. closcae were studied. The results showed that the optimum temperature for the degradation was 35℃ for the growth of the strain, the initial pH was 8.0, the conformable carbon source was fructose, the conformable nitrogen source was the yeast extract, the inoculum’s size was OD265:0.065, the density of dimethoate was 200mg/L, the air supply was 50ml/250ml volume in the cultural bottle with 180r/min speed in the rotated shaker, in which the degrading rates to dimethoate were ranged in 28.84%-61.19%. The L18（2*37）orthogonal test showed that the ion Cu2+and Fe2+ c promote the strain to degrade dimethoate, and Cd2+、Zn2+ could also promote it when it was at low level. However, Mg2+、Ba2+、Mn2+、Pb2+ had no effect on the degradation of dimethoate. Key Words: Enterobacter closcae, Dimethoate, degradation, optimize 农作物的生长过