楚雄师范学院甲醛对果蝇发育和繁殖的影响.docVIP

班级：11级葡萄酒班 学号：20111054101 甲醛对果蝇发育和繁殖的影响 摘要: 甲醛已被广泛用于人们的日常生活中，成为室内环境的主要污染源 为探讨甲醛对动物不同发育阶段的毒性，本文以模式生物果蝇 Drosophila melanogaster 为对象，研究了饲料中添加不同浓度甲醛对果蝇生长发育的影响 结果显示，当对照组发育至3 龄幼虫 ( 孵化后3 d) 时，与未添加甲醛的对照组相比，饲料中添加0. 25%或0. 50%的甲醛极显著降低了幼虫的体重 ( P ＜ 0. 01) ，添加了0. 50%甲醛的饲料组中发育出来的雌 雄成蝇体重也显著低于对照组，其中雌虫体重的差异达到极显著水平 此外，饲料中添加甲醛，还显著延长了幼虫发育的时间，即从胚胎到化蛹的发育时间 胚胎到羽化的发育时间都极显著长于对照组 ( P ＜ 0. 01) ，而其蛹期变态发育时间在对照组和各实验组之间没有显著差异 饲料中添加甲醛，还极显著降低了果蝇的产卵量 ( P ＜ 0. 01) 。综上结果表明，饲料中含有甲醛可显著降低果蝇的发育速度，影响果蝇的生长和繁殖力，但对果蝇蛹期变态发育过程没有显著影响。 关键词: 甲醛; 果蝇; 幼虫; 体重; 发育; 产卵 Effect of formaldehyde in diet on development and reproduction of Dro-sophila melanogaster TURSUN Abdurahman1， 2，ZHENG Ya1，WANG Yu- Feng1*( 1． Hubei Key Laboratory of Genetic Reg-ulation and Integrative Biology，College of Life Science，Huazhong Normal University，Wuhan 430079，China; 2． Department of Life and Environmental Science，Kashgar Teachers College，Kashgar 844000，China) Abstract: Formaldehyde is widely used in daily life nowadays． It has been the main source of environ-mental pollution caused by indoor decoration． To investigate the toxicity of formaldehyde on the develop-ment of different stages of Drosophila menlanogaster，we fed the flies with the diet containing formalde-hyde and observed the situations of their development and reproduction． We showed that the contamina-tion of 0. 25% or 0. 50% of formaldehyde resulted in significantly decreased body weight of the 3rdinstarlarvae ( P ＜ 0. 01) ，3- d- old female adults ( P ＜ 0. 01) and male adults ( P ＜ 0. 05) ． In addition，feed-ing with formaldehyde contaminated medium caused significantly longer developing time from embryo topupation than that in control ( P ＜ 0. 01) ，while the time of metamorphosis in pupae was not impacted．Formaldehyde also brought the significantly decreased amount of spawned eggs ( P ＜ 0. 01) ． These re-sults suggest that formaldehyde may inhibit the growth of larvae and adults，prolong the developmentaltime of fly larvae and impact on t