干细胞-骨髓间充质干细胞-三种大鼠骨髓间充质干细胞双向电泳样品制备方法的比较.pdfVIP

中国组织工程研究 第 16 卷 第 14 期 2012 –04 –01 出版 Chinese Journal of Tissue Engineering Research April 1, 2012 Vol.16, No.14 三种大鼠骨髓间充质干细胞双向电泳样品制备方法的比较***★ 覃万安，苏 伟，黄 钊，崔向荣，郭 杰 Three kinds of two-dimensional gel electrophoresis sample preparation methods of rabbit bone marrow mesenchymal stem cells Qin Wan-an, Su Wei, Huang Zhao, Cui Xiang-rong, Guo Jie Abstract Branch of Hand BACKGROUND: A variety of “standard” sample preparation methods for two-dimensional gel electrophoresis have been reported, Surgery, Department but there is no single one can be universally applied to rabbit bone marrow mesenchymal stem cells (rBMSCs). of Traumatic OBJECTIVE: To identif a suitable sample preparation method for rBMSCs two-dimensional gel electrophoresis (2-DE). Orthopedics, First Affiliated Hospital of METHODS: After full protein extraction of rBMSCs cultured in vitro, protein samples were divided into three groups: untreated, Guangxi Medical acetone precipitation and 2-D clean-up kit group, the protein concentration was measured by Bradford method, electrophoresis University, Nanning maps were analyzed. 530021,Guangxi RESULTS AND CONCLUSION: Using 2-D clean-up kit to treat the protein sample could obtain the best electrophoresis map Zhuang Autonomous which showed lowest background, clearest protein spots, highest resolution and good reproducibility. Protein extraction treated Region, China with 2-D Clean-up was a more suitable 2-DE sample preparation method of rBMSCs. Qin Wan-an★, Master, Resident Qin WA, Su W, Huang Z, Cui XR, Guo J. Three kinds of two-dimensional gel electrophoresis sample preparation methods of physician, Branch of rabbit bone marrow