Strain(Roltransgenemarker)：应变（rol基因的标记）.docVIP

Strain (Rol transgene marker) A?42 variant expressed relative A? expression P50 (hours) CL4176 wild type 1.0 22.77 ( 0.07 CL3523 G37L 1.28 ( 0.31 (32 CL3577 N27G G37L 1.45 ( 0.54 27.3 ( 0.25 CL3578 I31G G37L 2.21 ( 0.45 23.43 ( 0.24 CL4583 M35G G37L 2.45 ( 0.46 24.17 ( 0.52 Strain (GFP transgene marker) A?42 variant expressed relative A? expression P50 (hours) CL2659 wild type 1.0 18.77 (0.39 CL2621 G37L 0.89 ( 0.2 29.9 ( 0.38 CL2716 G37F 0.82 ( 0.16 29.93 ( 1.1 CL2666 G29L G33L G37L 1.16 ( 0.2 28.13 ( 0.99 CL2654 L17P 1.24 ( 0.2 19.93 ( 0.68 CL3743 N27G 2.69 ( 0.16 25.3 ( 0.89 CL2745 I31G 0.33 ( 0.58 19.0 ( 0.68 CL3748 M35G 0.37 ( 0.22 21.7 ( 0.53 Table S1. Relative A? expression of transgenic C. elegans strains. A? expression levels were assayed by immunoblot and calculated from three biologically independent samples for each transgenic strain. Normalization of A? variant expression to A? wild type was accomplished by pairing the appropriate A? wild type sample (i.e., from strain CL4176 or CL2659) on the same immunoblot as the variant strain sample. Monoclonal antibody 6E10, used to detect A? in all experiments, recognizes an N-terminal epitope in A? and that is not affected in any of the variants. P50 refers to the time required for 50% of the population to become paralyzed (average of three biologically independent experiments.)  Figure S1. Effects of dosage and preparation method on toxicity of A? wild type and A? G37L oligomers (ADDLs) in primary hippocampal cultures. Drebrin expression in cultured hippocampal cultures was quantified after exposure to fluorescently-tagged A??wild type oligomers (FADDLs), A??G37L oligomers, or mixtures of these peptides. The toxicity of FADDLs is similar to that of unlabeled A? wild type ADDLs (see Figure 6), and as observed for the unlabeled A? peptide, this toxicity is blocked by co-incubation of wild type and G37L peptides before addition to neurons (dark grey bar). In addition, prior exposure