钙蛋白酶10基因多态性与体重和胰岛素抵抗相关性.docVIP

钙蛋白酶10基因多态性与体重和胰岛素抵抗的相关性 摘要：目的 探讨钙蛋白酶10（CAPN10）基因SNP-19与体重和胰岛素抵抗（IR）的关系。方法：用PCR-RFLP法检测132名非超重肥胖和80名超重肥胖者的CAPN10基因SNP-19的基因型，测定身高、体重、空腹血糖（FPG）、血脂、胰岛素水平（FINS），计算体重指数（BMI）、腰臀比（WHR），用HOMA-IR法评价IR，并进行分析。结果：（1）BMI≥25kg/m2的个体有较高的FPG、WHR、TG和IR水平（p＜0.01），CAPN10基因SNP-19的22基因型和等位基因2的频率在BMI≥25kg/m2的个体明显升高（p＜0.05）；（2）携带基因型22的个体较基因型为11和12的个体有较高的FINS、BMI和IR水平。结论：CAPN10基因SNP-19基因型22可能与江苏地区人群超重肥胖的的发生有关。 关键词：钙蛋白酶10基因；单核苷酸多态性；体重指数；胰岛素抵抗； Relations among the CAPN10 gene and BMI, HOMA-IR Abstract: Objective: To explore the relations among SNP-19 of Calpain 10 gene and body mass index(BMI), insulin resistance(IR). Methods: Polymerase chain reaction-restrictive fragment length polymorphism( PCR-RFLP )was used to detected the genotype of site SNP-19 in calpain 10 gene. Fasting plasma glucose(FPG), insulin and IR were also studied. The people being tested include 132 non-overweight and obesity and 80 overweight and obesity. Results: (1) Higher FPG、WHR、TG and IR are found in the individuals with BMI≥25kg/m2 ,The frequency of 2 allele and 22 genotype of site SNP-19 in the individuals with BMI≥25kg/m2 was significantly higher than that in the controls with BMI＜25kg/m2; (p＜0.05). (2) Higher FINS、BMI and IR were found in the individuals with SNP-19 genotype 22 than those of individuals with genotype 11 and 12(p＜0.05). Conclusion: Polymorphism of site SNP19 of Calpain10 gene was related with overweight and obesity .There exited insulin resistance among the individuals with genotype 22 of SNP-19,which may be one of the genetic factors contributing to the occurrence of overweight and obesity. Keywords: Calpain 10 gene; Body mass index; Insulin resistance; single nucleotide polymorphism 钙蛋白酶10(CAPN10)基因是第一个被定位克隆的2型糖尿病的易感基因，国内外学者大多致力于其与2型糖尿病关系方面的研究。鉴于2型糖尿病与超重肥胖常伴随发生，且都是多基因遗传病，有共同的发病基础—胰岛素抵抗，本文对钙蛋白酶10基因SNP-19位点的多态性与超重肥胖、胰岛素抵抗的关系进行了研究。 一. 研究对象与方法 1 . 研究对象 随机抽取在江苏大学附属医院体检的健康查体者及门诊就诊的2型糖尿病患者共212例，按照1997年WHO标准体重指数(BMI)≥25kg/m2为超重及肥胖（80例），BMI＜25kg/m2为非超重肥胖（132例），