镉诱导大鼠肾细胞凋亡及机理研究.pdfVIP

2008 3 Vol.3, 2008 3 , 268−273 Asian Journal of Ecotoxicology No.3, 268−273 镉诱导大鼠肾细胞凋亡及其机理的研究 * 旻 贾广乐 ，王众，吴绍强，林祥梅，韩雪清，刘建，梅琳，张 中国检验检疫科学研究院，北京 100029 摘要(Cd)(NRK cell)010204060μmol·L− 1 CdCl2 NRK 6h. NRK Caspase-8 RT-PCR Caspase-8Fas mRNA . CdCl2 NRK Caspase-8 Caspase-8Fas mRNA -. NRK . 关键词NRK 文章编号1673-5897(2008)3-268-06 中图分类号Q813 文献标识码A Study on the Mechanisms of NRK Cells Apoptosis Induced by Cadmium * JIA Guang-le , WANG Zhong, WU Shao-qiang, LIN Xiang-mei, HAN Xue-qing, LIU Jian, MEI Lin, ZHANG Min The Chinese Academy of Inspection and Quarantine, Beijing 100029 Received 2 February 2008 accepted 23 March 2008 Abstract: In order to study the death-receptor pathway in NRK cell apoptosis induced by cadmium, different concentrations of cadmium chloride (0, 10, 20, 40, 60μmol·L− 1) were added to NRK cell culture medium and cultured for 6h. Apoptosis rate, Caspase-8 activity and expression level of mRNA of Caspase-8 and Fas were detected by flow cytometry, spectrophotometer and RT-PCR, respectively. Results showed that concomitant dose-dependent increases in apoptosis rate, Caspase-8 activity and the expression level of Caspase-8 and Fas mRNA were found, which indicated apoptosis of NRK cells could be induced by cadmium through death-receptor pathway. Keywords: cadmium; NRK cell; death-receptor pathway 收稿日期: 2008-02-02 录用日期: 2008-03-23 基金项目: (No. 2007JK022) 作者简介: (1973—)*通讯作者(Corresponding author)E-mail: gljiasx@ 3 : 269 1 引言(Introduction)