β-榄香烯对肾细胞癌的抗肿瘤作用及机制研究.pdfVIP

2012 1 20 1 MODERN ONCOLOGY ，Jan. 2012 ，VOI. 20 ，NO. 01 ·1 · 现代肿瘤医学 年 月 第 卷第 期 ?论 著? ?基础研究? β － 榄香烯对肾细胞癌的抗肿瘤作用及机制研究 1 1 2 2 2 2 1 ， ， ， ， ， ， 詹运洪 王科峰 刘 静 曲秀娟 刘云鹏 侯科佐 吴 斌 The anti － tumor effect of β － elemene on renal － cell carcinoma cells and the mechanisms involved 1 1 2 2 2 2 1 ZHAN Yunhong ，WANG Kefeng ，LIU Jing ，QU Xiujuan ，LIU Yunpeng ，HOU Kezuo ，WU Bin 1 2 Dep artment of Urology ，Shengj ing Hosp ital of China Medical University ，Liaoning Shenyang 110004 ，China ;Dep artment of Medical Oncology ，The First Hosp ital of China Medical University ，Liaoning Shenyang 110001，China． 【Abstract 】 Objective :To investigate the anti － tumor effect of β － elemene on renal － cell carcinoma (RCC)786 － 0 cells and the mechanisms involved． Methods :786 － 0 cells were treated with different concentrations of β － el- emene for 24 ，48 or 72 h． Cell viability was determined using MTT assay． Apoptosis and cell cycle distribution were determined with flow cytometry following Propidium iodide (PI)staining． Expression of apoptosis associated proteins was detected by western blot． Results : － Elemene inhibited the viability of 786 － 0 cells in a dose － and time － de- β pendent manner． Flow cytometry assay showed that β － elemene induced apoptosis ，but had little effect on cell cycle distribution． Western bolt showed that β － elemene cleaved PARP and down － regulated the expression of Bcl － 2 and Survivin． Conclusion :The anti － tumor effect of β － elemene on RCC 786 － 0 cells might be asso