活性玉米醇溶蛋白肽分离纯化及还原活性的研究.pdfVIP

食 品 科 技 粮食与油脂 FOOD SCIENCE AND TECHNOLOGY 2011 36 6 年 第 卷 第 期 活性玉米醇溶蛋白肽的分离纯化及其 还原活性研究 李鸿梅，高名利，张 路 (吉林农业大学食品科学与工程学院，长春 130118) 摘要：碱性蛋白酶水解玉米醇溶蛋白，精制后的水解产物为玉米醇溶蛋白肽。根据玉米醇溶蛋 白肽对邻苯三酚自氧化的抑制活性，采用葡聚糖凝胶层析法及高效液相层析(HPLC)法分离纯化 得到活性蛋白肽的几种组分，探讨了几种组分对超氧阴离子自由基的清除活性。同时，以α－ 生育酚为阳性对照，探讨了活性蛋白肽及其各组分的还原力。结果表明，经Sephedax G-10葡 聚糖凝胶层析分离所得组分4的抗氧化活性最强，具有较高的还原力；组分4富含谷氨酸、丙氨 酸、亮氨酸、组氨酸、脯氨酸和苯丙氨酸。 关键词：玉米醇溶蛋白肽；抗氧化活性；分离纯化；还原活性 中图分类号：TS 210.9 文献标志码：A 文章编号：1005-9989(2011)06-0190-05 Separation and purifi cation of active zein peptide and their reducing powers LI Hong-mei, GAO Ming-li, ZHAN Lu (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118) Abstract: Zein was hydrolyzed with an alkaline protease. The hydrolysate was refi ned and called zein peptide. Zein peptide was separated and purifi ed by Sephedax column and high-performance liquid chromatography (HPLC) method based on its inhibition effect on pyrogallol auto-oxidation. The scavenging effect of active zein peptide and its fractions on superoxide anion radical were researched. Their reducing powers were also studied while using α-tocopherol as the positive control. The data revealed that the activest fraction on antioxidant ability and reducing power was fraction 4 separated by Sephadex G10 column. Glutamic acid, alanine, leucine, histidine, proline and phenylalanine are rich in fraction 4. Key words: zein peptide; antioxidant activity; separation and purifi cation; reducing power 机体发生酶促反应或非酶促反应过程中会产 反应生成其他自由基。这些自由基的积累会对机 生过氧化氢、超氧阴离子、羟自由基、单线态氧