应用基因芯片方法检测结核分枝杆菌利福平和异烟肼耐药性.pdfVIP

80 2011 10 33 10 Chin J Antituber c, October 2011, Vol33, No10 张俊仙 吴雪琼 阳幼荣 梁艳 ( My coba cterium tuberculosis , Mtb) , (polymerase chain reaction, PCR) - 30 M tb 50 rp oB ka tG inhA , PCR- PCR-30 Mtb rp oB katG inhA 50 M tb , PCR-3 rp oB , 41 ; PCR- 50 Mtb , PCR- 1 katG 30 inhA , 31 ka tG 315 , 7 inhA - 15 , 2 katG inhA ; 3 ka tG 13 inhA PCR- PCR- Mtb , , ; ; ; , ; Rapid detection of Mycobacterium tubercu losis resistance to rifampicin and isoniazid by gene chip ZHANG Jun- xian , WU X ue- iong , YANG You-r ong , LIAN G Yan . Army Tuberculosis Preven tion an d Con trol K ey Laboratory , th Institutef or Tuberculosis Research , The 309 Hosp ital of Chin ese Peop leL iberation Army , Beij ing 100091, China Corresp on ding author : WU X ue- iong , Em ail :wu-xue iong @263 . n et Abstract Objective T o detect My cobacterium tuberculosis ( Mtb) resist ance to rifampicin ( RIF) and isoniazid ( INH) by gene chip, and to evaluate their clinical values. Methods T hirt y RIF- and INH-susceptible and 50 RIF- and INH-resistant clinical isolat es in M tb w ere analyzed the mutations of rp oB katG and inhA genes by ( PCR)-gene chip and DNA sequencing. Results The rp oB ka tG and inhA genes from 30 RIF- and INH-susceptible isolates of Mtb w ere all w ild types by PCR-gene chip and DNA sequencing . Of 50 RIF-resistant clinical isolates, 3 had wild types of ropB genes, and 4 1 had ropB mutations by PCR-gene chip and DNA sequencing. Six had inconsistent re- sults between PCR-gene chip and DNA sequencing . Of 50 INH- resistant clinical isolates, 1 had wild types of katG genes, and 31 had katG mutations on codon 315; 30 had w ild types of inhA genes, and 7 had inhA m