鹰嘴豆锌指蛋白基因ZF1克隆及表达分析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2009, 35(12): 2180−2186 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.02180 鹰嘴豆锌指蛋白基因ZF1 的克隆及表达分析 陈 晨1 彭 辉1 高文瑞1 石庆华2 张 桦2 张巨松2 李建贵2 麻 浩1,* 1 / , 210095; 2 , 830052 : PEG cDNA EST , 3′RACE C2H2 ZF1, , 244 , Cys2/His2 , GFP , ZF1 RT-PCR , ZF1 , , RT-PCR PCR , ZF1 , 6-(6-BA)(ABA)(Et)(GA3)-3-(IAA)(MeJA) (SA), ZF1 : ; ; ; ; Cloning and Expression of Zinc Finger Protein Gene ZF1 in Chickpea (Cicer arietinum L.) 1 1 1 2 2 2 2 CHEN Chen , PENG Hui , GAO Wen-Rui , SHI Qing-Hua , ZHANG Hua , ZHANG Ju-Song , LI Jiang-Gui , and MA Hao1,* 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement / Soybean Research Institute, Nanjing Agricultural University, Nanjing 210095, China; 2 Key Laboratory of Agricultural Biotechnology, Xinjiang Agricultural University, Urumqi 830052, China Abstract: Regulation of gene expression at the level of transcription controls many crucial biological processes including growth and development, stress response, signal transduction and disease resistance. A number of factors, such as C2H2 zinc finger protein, are required and factors play an important role in the transcription. Chickpea (Cicer arietinum L.) is the third important legume crop gown mainly in the arid and semi-arid reg