miR398在植物逆境胁迫应答中作用.pdfVIP

HEREDITAS (Beijing) 2010 2 , 32(2): 129―134 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2010.00129 miR398 在植物逆境胁迫应答中的作用 1 2 3 1 丁艳菲, 王光钺 , 傅亚萍 , 朱诚 1. , 310029; 2. , 311300; 3. , 310006 MicroRNA (miRNA)是一类新型的调控基因表达的小分子 RNA, 它作为基因表达的负调控因子, 在转录 后水平调节靶基因的表达。miRNA 参与调控植物的生长发育, 并在多种非生物与生物胁迫响应中发挥重要作 用。miR398 是第一个被报道的受氧化胁迫负调控的 miRNA 。它通过负调控其靶基因 Cu/Zn 过氧化物歧化酶 (Cu/Zn-superoxide dismutase, CSD) 的表达, 在多种逆境胁迫响应中扮演重要角色, 如调节铜代谢平衡, 应答重 金属、蔗糖、臭氧等非生物胁迫, 以及参与应答生物胁迫等。文章综述了miR398 在多种逆境胁迫响应中重要 的调节作用及miR398 自身的转录调控。 miR398; 非生物胁迫; 生物胁迫; 基因表达调控; 胁迫应答 The role of miR398 in plant stress responses 1 2 3 1 DING Yan-Fei , WANG Guang-Yue , FU Ya-Ping , ZHU Cheng 1. College of Life Sciences, Zhejiang University, Hangzhou 310029, China; 2. College of Forest and Biotechnology, Zhejiang Forestry University, Lin’an 311300, China; 3. China National Rice Research Institute, Hangzhou 310006, China Abstract: MicroRNAs (miRNAs) are a new class of small RNAs, which act as post-transcriptional negative regulators of gene expression. Plant miRNAs are important in the regulation of plant growth, development and in response to various abiotic and biotic stresses. miR398 is the first reported miRNA to be down-regulated by oxidative stresses. miR398 plays an important role in stresses, such as regulating copper homeostasis, in response to abiotic stresses including heavy metals, sucrose, and ozone and biotic stresses via down-regulating the expression of Cu/Zn-superoxide dismutase (CSD). This review focused on the crucial role of miR398 in regulation of different stresses and the transcriptional r