变应性疾病患者血清IL-4、sCD23研究.docxVIP

变应性疾病患者血清IL-4、sCD23的研究变应性疾病患者血清IL-4、sCD23的研究中华皮肤科杂志 1998年第6期第31卷 论著作者：张开明　李秉煦　唱文娟　尹兴平　杨小英　周敏单位：030009 太原市中心医院皮肤科(张开明　尹兴平　唱文娟　杨小英　周敏)；温州医学院第一附属医院皮肤科(李秉煦)关键词：荨麻疹；湿疹；鼻炎；白细胞介素4；受体；IgE【摘要】　目的　为了解几组变应性疾病患者血清IL-4和sCD23水平及IL-4对sCD23(CD23)产生的影响。方法　采用ELISA法对荨麻疹、湿疹及过敏性鼻炎患者血清、外周血单一核细胞(PBMC)及培养条件下IL-4、sCD23(CD23)水平及调控关系进行了研究。结果　13组疾病血清IL-4及sCD23水平均明显高于正常人对照组(P＜ 0.05～0.01)，sCD23与IL-4之间具有明显的正相关关系(r=0.68～0.92，P＜ 0.01)；2rIL-4可促进PBMC产生和表达sCD23(CD23)，湿疹患者rIL-4对PBMC产生sCD23的诱导能力比正常对照组明显增强。结论　IL-4及sCD23参与了变应性疾病的发展过程，IL-4诱导PBMC产生sCD23为时间效应关系。Study on Serum Levels of IL-4 and sCD23 in Patients with Allergic Diseases Zhang Kaiming, Li Binxu, Chan Wenjuan, et al. Department of Dermatology, Taiyuan Central Hospital, Taiyuan 030009【Abstract】　Objective　To investigate the serum levels of IL-4 and sCD23 in patients with allergic diseases and the effect of IL-4 to the production of sCD23.Methods The levels of IL-4 and sCD23 in the sera and peripheral blood mononuclear cells (PBMC) from patients with urticaria, eczema and rhinitis, and the level of CD23 in the cultured PBMC with and without exogenous IL-4 were measured by ELISA.Results　(1) The levels of IL-4 and sCD23 were higher in patients with allergic diseases than those in controls,and there were positive correlation between IL-4 and sCD23 (r=0.68～0.92, P＜ 0.01); (2) rIL-4 could promote the production and expression of sCD23 (CD23) in PBMC, this effect was much more remarkable in PBMC from patients with eczema than that from controls.Conclusion The results confirm that IL-4 and sCD23 play a role in the pathogenesis of allergic diseases.【Key words】　Urticaria　Eczema　Rhinitis　Interleukin-4　Receptors, IgE近年的研究发现IL-4和CD23与变态反应性疾病密切相关，两者相互协调在变态反应中起着重要的作用。为此，我们采用ELISA法及APAAP法对荨麻疹、湿疹、遗传过敏性皮炎和过敏性鼻炎患者血清、外周血单一核细胞(PBMC)及培养条件下的IL-4、sCD23(CD23)和总IgE水平及调控关系进行了研究，现将结果报道如下。资料与方法一、临床资料检测对象：病例为1996年8月至1997年5月皮肤科门诊和住院的患者，共149例，其中荨麻疹68例(急性荨麻疹27例，病程＜ 4周；慢性荨麻疹41例，病程 ＞4周)，在慢性荨麻疹患者中，分别于缓解期(无明显风团29例)和发疹期(30例)采血；湿疹43例(原因不明的原发性湿疹， 不包括其它皮肤病湿疹化的患者)；遗传过敏性皮炎14例；过敏性鼻炎24例(其中15