硫化氢对家兔窦房结起搏细胞电生理效应.pdfVIP

Acta Physiologica Sinica , April 25, 2008, 60 (2): 175-180 175 Research Paper Electrophysiological effects of hydrogen sulfide on pacemaker cells in sinoatrial nodes of rabbits * XU Meng, WU Yu-Ming , LI Qian, WANG Xin, HE Rui-Rong Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazh uang 050017, China Abstract: The cardiac electrophysiological effects of hydrogen sulfide (H2 S) on pacemaker cells in sinoatrial (SA) nodes of rabbits were examined using intracellular microelectrode technique. The results obtained were as follows: (1) The velocity of diastolic (phase 4) depolarization (VDD) and rate of pacemaker firing (RPF) in normal pacemaker cells in SA nodes were decreased by NaHS (H2 S donor) (50, 100, 200 µmol/L) in a concentration-dependent manner; (2) ATP-sensitive K+ (KATP) channel blocker glybenclamide (Gli, 20 µmol/ L) blocked the effect of NaHS (100 µmol/L) on pacemaker cells; (3) Pretreatment with CsCl (2 mmol/L), a blocker of pacemaker current (If), did not affect the effect of NaHS (100 µmol/L) on SA node pacemaker cells; (4) DL -propargylglycine (PPG, 200 µmol/L), an inhibitor of cystathionine γ-lyase (CSE), did not affect the parameters of action potentials in pacemaker cells in SA nodes. All these results suggest that H2 S exerts a negative chronotropic action on pacemaker cells in SA nodes of rabbits. These effects are likely due to an increase in potassium efflux through opening K channels; I is unlikely to play a major role in these effects. In our study, there was ATP f no evidence for the generation of endogenous H2 S by CSE in SA node pacemaker cells. Key words: electrophysiology; hydrogen sulfide; action potential; sinoatrial node 硫化氢对家兔窦房结起搏细胞的电生理效应 * 许 萌，武宇明 ，李茜，王昕，何瑞荣 河北医科大学基础医学研究所生理室，石家庄 05