滑菇多醣體成分分析與免疫調節活性之探討.docVIP

滑菇多醣體成分分析與免疫調節活性之探討 Component analysis and immunomodulatory activities of polysaccharide isolated from Pholiota nameko 研究生：賴景瑋 Lai, Jing-Wei 指導教授：吳美莉 Wu, Mei-Li 【摘要】 食品和醫藥用的菇類具有營養和豐富的生理活性物質，是現今開發保健食品的重要來源。在許多文獻中指出菇類多醣體具有抗腫瘤以及免疫活性。本研究利用熱水和95%酒精萃取滑菇多醣。其多醣萃取物之總糖含量以酚硫酸法測定；所測得的總糖含量分別為65.92%。從滑菇分離出來的粗萃多醣體利用Sephacryl S-400 HR管柱純化過後可得到三個純化多醣體，分別為PNFA、PNFB、PNFC。使用高效能液相層析儀(HPLC)搭配膠體過濾管柱測得其三個純化多醣體的分子量分別為1526.7 kDa、19.5 kDa、3.7 kDa。將這三個純化的多醣體作用於自然殺手細胞 (NK cell)和小鼠淋巴瘤細胞 (Yac-1)觀察免疫調節的活性。結果顯示，純化的多醣體皆可以提高自然殺手細胞的增生，還能提高自然殺手細胞對小鼠淋巴瘤細胞之細胞毒殺作用的活性，其中效果最好的為PNFC (0.266 ± 0.035；70.07 ± 4.21%)。因此從滑菇子實體分離出的純化多醣體將來可應用在免疫調節藥物治療上的藥劑。 滑菇、多醣體、細胞毒殺作用Edible and medicinal mushrooms are nutritious and rich in physiologically active substances, which are important materials for the health food industry. Accumulated studies indicated that mushroom polysaccharides possess antitumor and immunostimulating activities. In the present study, polysaccharides were extracted from Pholiota nameko by hot aqueous extraction and precipitation with 95% ethanol. Total carbohydrate contents were estimated by the phenol-sulfuric acid method, and it was indicated that 65.92% of the total carbohydrates were contained in the polysaccharide preparations. The crude polysaccharides isolated from P. nameko was further separated into three fractions (PNFA, PNFB and PNFC ) using an Sephacryl S-400 HR column. Their molecular weight distributions were determined using high performance liquid chromatography (HPLC) with gel filtration column. The molecular weight of these three polysaccharide preparations were determined to be 1526.7 kDa , 19.5 kDa, and 3.7 kDa. These fractions were tested for in vitro immunomodulatory effects on Yac-1 cell model. All of the three polysaccharide preparations elevated natural killer (NK) cell proliferation and cytotoxicity activity. Thus, the three fraction polysaccharides derived from the P. nameko fruit body could be used as immunomodulators agents. Keywords：P. nameko, polysaccharide