多肿瘤标志物蛋白芯检测系统在临床应用中的评价.docVIP

多肿瘤标志物蛋白芯片检测系统在临床应用中的评价 李心河1，单宁宁1，2,高扬1，王惠敏1，刘延红1，夏洪印1，邹雄1，侯明2 1. 山东大学齐鲁医院检验科中心实验室，济南，250012 2. 山东大学齐鲁医院血液科，济南，250012 [摘要]目的:评价多肿瘤标志物蛋白芯片在健康人群的普查、肿瘤的早期诊断及监测预后的应用。方法:使用HD-2OO1A多肿瘤标志物蛋白芯片检测系统（C-12）测定分析8330例恶性肿瘤患者，9657例良性疾病患者和23531例健康体检者血清12种常见肿瘤标志物(CA199，NSE，CEA，CA242，CA125，CA153，AFP，FER，f-PSA，PSA，β-HCG及HGH)的水平。同时对恶性肿瘤组的153例阴性标本用E170全自动免疫分析仪进行比对分析。结果:恶性肿瘤组的阳性率(85.6%)显著高于良性疾病组(41.2%)和健康体检组(7.03%)(P0.05)。C-12对健康人群检测的特异性为95%，对健康人群肿瘤的检出率为0.2%。结论:多肿瘤标志物蛋白芯片检测技术具有较高灵敏度和特异性。运用蛋白芯片技术联合检测多种肿瘤标志物可以明显提高恶性肿瘤诊断的敏感性，可应用于预后及疗效观察，同时也可以作为无症状人群的早期肿瘤普查手段之一，对肿瘤高危人群的普查具有一定的指导意义。 关键词:肿瘤标志物;蛋白芯片；化学发光分析 Evaluation of Clinical Application of Detective System of Multi-tumor Markers Protein Chip Li Xin-he1,Shan Ning-ning1，2,Gao Yang1,Wang Hui-min1,Liu Yan-hong1,Xia Hong-yin1，Zou Xiong1，Hou Ming2 (1.Clinical Laboratry of QiLu Hospital, Shandong University,Jinan 250012 China 2. Haematology Oncology Centre, Qilu Hospital, Shandong University, Jinan 250012, China) [ABSTRACT]Objective:Identification of clinical applications to cancer diagnosis with multi-tumor markers protein chip and its speeificity and sensitivity in detection of non-caneer subjeets and healthy controls. Methods:The serum levels of 12 tumor markers，includingCA199，NSE，CEA，CA242，CA125，CA153，AFP，Ferritin，Free一PSA，PSA，β-HCG and HGH ----------------------------------------- [通讯作者] 邹雄(1955 - ) ,男, 教授,博士研究生导师,研究方向:肿瘤免疫。 电话：0531 e-mail: zx2186@21 benign disease and 23531 healthy examinations.Results:The Positve rates were measured with the detective system in 8330 malignant tumor Patients，9657 Patients with were(85.6%)in malignant tumor，(41.2%)benign disease and(7.03%)healthy groups，respectively.The malignant tumor group had signifieantly higher positive rate than that of the controls.Conclusion:Combined measurement of multiple serum tumor markers using protein biochip technique can significantly increase the diagnostic sensitivity for malignant tumor，and can also be used as an earlier tumor-screening tool