成人骨髓间充质干细胞对脐血CD34+细胞的体外扩增作用_医学论文.docVIP

成人骨髓间充质干细胞对脐血CD34+细胞的体外扩增作用_医学论文 成人骨髓间充质干细胞对脐血CD34+细胞的体外扩增作用_医学论文 作者：贾明峰,侯相麟,赵丽,刘蓓,李娟,陈轩 【关键词】 骨髓细胞 　　【Abstract】 AIM: To investigate the effects of adult bone marrow mesenchymal stem cells on the expansion of umbilical cord blood CD34+ cells ex vivo. METHODS: Adult bone marrow mesenchymal stem cells were isolated and cultured as feeder layer. Umbilical cord blood CD34+ cells were cultured in combination with mesenchymal stem cells, stem cell factor, Interleukin11 and granulocytemacrophage colony stimulating factor, respectively. At the end of 3 weeks, the total number of nucleated cells , colony forming cells and CD34+ cells was repeatedly counted in culture system for 3 weeks to obtain the fold of expansion. RESULTS: The mesenchymal stem cells and cytokines coculture systems significantly enhanced the expansion of the cord blood cells with the increase of the total number of nucleated cells by 114.2±2.4fold, colony forming cells by 40.5±8.6fold and CD34+ cells by 11.3±0.4fold, respectively. CONCLUSION: The expansion of umbilical cord blood CD34+ cells can be enhanced ex vivo by adult bone marrow mesenchymal stem cells combined with cytokines. 　　【Keywords】 ex vivo amplification CD34+ cell cord blood bone cells stem cells 　　【摘要】 目的： 探讨骨髓间充质干细胞(MSCS)对脐血CD34+细胞体外扩增作用. 方法： 分离培养成人MSCS作为滋养层，联合SCF, IL11和GMCSF分别组成对脐血CD34+细胞的不同扩增体系，培养3 wk后观察脐血有核细胞、CFCs以及CD34+细胞扩增倍数的变化. 结果： MSCS联合细胞因子组较其他组培养体系对有核细胞总数、CFCs含量及CD34+细胞含量均具有明显的扩增作用，分别扩增了114.2±2.4, 40.5±8.6, 11.3±0.4 倍. 结论： 成人MSCS协同其他细胞因子可增强脐血CD34+细胞的体外扩增作用. 　　【关键词】 体外扩增；CD34+细胞；脐血；骨髓细胞；干细胞 　　0引言 　　骨髓间充质干细胞（mesenchymal stem cells, MSCs）作为骨髓造血微环境中的一种重要细胞成分,近年来成为干细胞研究领域的一大热点. MSCs具有自我更新和多向分化潜能，在体外可被诱导分化为多种结缔组织及部分来源于外胚层的组织，形成骨、软骨、骨骼肌、腱、韧带、真皮、脂肪、骨髓基质和神经［1,2］. 此外，还分泌多种生长因子支持骨髓造血，藉此MSCs可在体外作为滋养细胞层进行造血干/祖细胞扩增. 自脐血（umbilical cord blood,UCB）中发现造血干细胞(hematopoietic stem cell,HSC)以来，脐血HSC移植用于治疗恶性及非恶性血液病日益广泛，但单份脐血所含的造血细胞不足以满足成人的移植所需，大量扩增后才能用于移植. 鉴于此，我们探讨了来自成人的MSCS对脐血CD34+ 细胞的体外扩增作用. 　　1材料和方法 　　1.1材料 　　正常成年男性献骨髓者2例，健康足月分娩儿脐血14例,取自兰州市慈和堂